Widespread Molecular Detection of Legionella pneumophila Serogroup 1 in Cold Water Taps across the United States
Widespread Molecular Detection of Legionella pneumophila Serogroup 1 in Cold Water Taps across the United States
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DOI：
10.1021/es4055115
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发表时间：
2014-03-18
影响因子：
11.4
通讯作者：
Pfaller, Stacy
中科院分区：
文献类型：
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作者：
Donohue, Maura J.;O'Connell, Katharine;Pfaller, Stacy
在美国，2009-2010年向疾病控制和预防中心报告了6，868例军团菌病。在这些报告中，估计84%是由微生物嗜肺军团菌血清群（Sg）1引起的。军团菌属从各种天然淡水环境中分离和回收。人体暴露于L.嗜肺菌Sg 1可能因家庭和设施用水的雾化和随后的吸入而发生。在这项研究中，两个引物/探针组（一个能够检测L。pneumophila和L. pneumophila Sg 1）对它们各自的靶标具有高度敏感性和选择性。2009年和2010年从美国68个公共和私人水龙头收集了超过272个水样，使用两种qPCR检测方法进行分析，以评估L.嗜肺菌Sg 1.近一半的水龙头显示L的存在。在一次采样事件中，16%的水龙头在一次以上的采样事件中呈阳性。这项研究是美国第一个记录L. pneumophila Sg 1在从使用点水龙头输送的冷水中。
In the United States, 6,868 cases of legionellosis were reported to the Center for Disease Control and Prevention in 2009-2010. Of these reports, it is estimated that 84% are caused by the microorganism Legionella pneumophila Serogroup (Sg) 1. Legionella spp. have been isolated and recovered from a variety of natural freshwater environments. Human exposure to L. pneumophila Sg1 may occur from aerosolization and subsequent inhalation of household and facility water. In this study, two primer/probe sets (one able to detect L. pneumophila and the other L. pneumophila Sg1) were determined to be highly sensitive and selective for their respective targets. Over 272 water samples, collected in 2009 and 2010 from 68 public and private water taps across the United States, were analyzed using the two qPCR assays to evaluate the incidence of L. pneumophila Sg1. Nearly half of the taps showed the presence of L. pneumophila Sg1 in one sampling event, and 16% of taps were positive in more than one sampling event. This study is the first United States survey to document the occurrence and colonization of L. pneumophila Sg1 in cold water delivered from point of use taps.