Radioimmunoassay of rhesus monkey follicle stimulating hormone (RhFSH).
Radioimmunoassay of rhesus monkey follicle stimulating hormone (RhFSH).
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恒河猴促卵泡激素（RhFSH）的放射免疫测定。
DOI：
10.1210/endo-92-6-1652
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发表时间：
1973
期刊：
影响因子：
4.8
通讯作者：
E. Knobil
中科院分区：
文献类型：
--
作者：
T. Yamaji;W. Peckham;L. Atkinson;D. Dierschke;E. Knobil
建立了两种测量猕猴卵泡刺激素（RhFSH）的放射免疫测定法。纯化的RhFSH制剂的生物效力为200 U/mg，在用131I碘化后用作两种测定的痕量。其中一种系统，即同源测定，使用豚鼠的抗血清，该血清用恒河猴脑垂体的粗糖蛋白制剂免疫。用纯化的猕猴促甲状腺激素（RhTSH）吸收该抗血清后，同源检测对RhFSH具有特异性。在另一种系统中，可以利用两种人卵泡刺激素抗血清（anti-hFSH）中的任何一种进行异源检测。该试验对RhFSH及其2亚基均具有特异性。这两种方法的敏感性足以测量恒河猴正常月经周期的所有阶段的卵泡刺激素。（内分泌学92:1652,1973）
Two radioimmunoassays for the measurement of rhesus monkey (Macaca mulatto) follicle stimulating hormone (RhFSH) have been developed. A purified RhFSH preparation with a biological potency of 200 U/mg is utilized as trace in both assays following its iodination with 131I. One of the systems, the homologous assay, employs antiserum from a guinea pig which had been immunized with a crude glycoprotein preparation from rhesus monkey pituitary glands. After absorption of this antiserum with purified rhesus monkey thyroid stimulating hormone (RhTSH), the homologous assay is specific for RhFSH. In the other system, the heterologous assay, either of two antisera to human FSH (anti—hFSH) can be utilized. This assay is specific for both RhFSH and its ² subunit. The sensitivities of both assays are sufficient to measure circulating FSH at all phases of most normal menstrual cycles in the rhesus monkey. (Endocrinology 92: 1652, 1973)