Axon fasciculation and differences in midline kinetics between pioneer and follower axons within commissural fascicles
Axon fasciculation and differences in midline kinetics between pioneer and follower axons within commissural fascicles
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DOI：
10.1242/dev.00713
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发表时间：
2003-10-01
期刊：
影响因子：
4.6
通讯作者：
Fraser, SE
中科院分区：
文献类型：
--
作者：
Bak, M;Fraser, SE
早期神经元支架发育研究表明，最初的神经元及其轴突为后来的神经元及其过程提供指导。虽然这种排列可能有助于轴突导航，但这种相互作用的具体后果在体内是未知的。我们使用延时荧光显微镜跟踪活体斑马鱼胚胎前脑连合的形成，以定量检查中线处的连合轴突动力学：轴突相互作用可能很重要的地方。虽然通常认为连合轴突在中线处减慢，但我们的数据显示这仅适用于领导轴突。跟随轴突不显示这种行为。然而，当领导轴突消融，跟随轴突改变其中线动力学和领导者的行为。类似地，当对侧引导轴突沿着沿着相反的引导轴突穿过中线生长时，对侧引导轴突改变它们的中线动力学。这些数据表明一个简单的模型，其中生长锥暴露于中线线索的水平和存在的其他轴突作为基板形状的连合轴突的中线动力学。
Early neuronal scaffold development studies suggest that initial neurons and their axons serve as guides for later neurons and their processes. Although this arrangement might aid axon navigation, the specific consequence(s) of such interactions are unknown in vivo. We follow forebrain commissure formation in living zebrafish embryos using timelapse fluorescence microscopy to examine quantitatively commissural axon kinetics at the midline: a place where axon interactions might be important. Although it is commonly accepted that commissural axons slow down at the midline, our data show this is only true for leader axons. Follower axons do not show this behavior. However, when the leading axon is ablated, follower axons change their midline kinetics and behave as leaders. Similarly, contralateral leader axons change their midline kinetics when they grow along the opposite leading axon across the midline. These data suggest a simple model where the level of growth cone exposure to midline cues and presence of other axons as a substrate shape the midline kinetics of commissural axons.