Reduced Transplacental Transfer of a Subset of Epstein-Barr Virus-Specific Antibodies to Neonates of Mothers Infected with Plasmodium falciparum Malaria during Pregnancy
Reduced Transplacental Transfer of a Subset of Epstein-Barr Virus-Specific Antibodies to Neonates of Mothers Infected with Plasmodium falciparum Malaria during Pregnancy
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DOI：
10.1128/cvi.00270-15
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发表时间：
2015-11-01
影响因子：
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通讯作者：
Rochford, Rosemary
中科院分区：
文献类型：
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作者：
Ogolla, Sidney;Daud, Ibrahim I.;Rochford, Rosemary
在疟疾流行地区，超过35%的儿童在6个月大时感染了EB病毒（EBV）。这种易感性可能与抗体经胎盘转移受损有关。在这项研究中，我们确定了肯尼亚西部疟疾流行地区怀孕期间接触疟疾对EB病毒特异性母体抗体转移的影响。招募孕妇并对其进行跟踪，直至分娩，以确定新生儿疟疾暴露水平。使用基于Luminex珠的测定法，在70对母体和脐带血样本中测量EBV裂解性（病毒衣壳抗原[VCA]、Z转录激活因子[Zta]和早期弥漫性抗原复合物[EAd]）和EBV潜伏性（EBV核抗原-1（EBNA 1））以及破伤风特异性IgG抗体的水平。很高比例（63%）的婴儿在子宫内接触疟疾。感染疟疾的母亲和没有检测到疟疾感染的母亲的EB病毒和破伤风特异性抗体水平相似。疟疾暴露的新生儿有显着较低水平的抗EBNA 1，抗Zta，抗EAd抗体比他们的母亲。子宫内疟疾暴露导致抗VCA-p18和抗EBNA 1抗体的经胎盘转移分别显著减少13%和22%。新生儿收到显着低水平的抗Zta和抗EAd抗体，无论疟疾暴露水平。在多变量分析中，子宫内疟疾暴露与新生儿抗VCA-p18和抗EBNA 1抗体转移显著减少相关（分别为P = 0.0234和P = 0.0017）。妊娠期间的疟疾导致EBV特异性抗体从母亲转移到胎儿的差异水平。某些抗体经胎盘转移受损可能导致疟疾暴露的新生儿易受早期EBV感染。
Over 35% of children in a region of malaria endemicity are infected with Epstein-Barr virus (EBV) by 6 months of age. This susceptibility may be linked to impaired transplacental transfer of antibodies. In this study, we determined the effect of malaria exposure during pregnancy on the transfer of EBV-specific maternal antibodies in a region of western Kenya that experiences endemic malaria. Pregnant mothers were recruited and followed up until delivery to determine levels of neonatal malaria exposure. Levels of EBV lytic (viral capsid antigen [VCA], Z transcriptional activator [Zta], and early diffuse antigen complex [EAd]) and EBV latent (EBV nuclear antigen-1 (EBNA1]) and tetanus-specific IgG antibodies were measured in 70 paired maternal and cord blood samples using a Luminex-bead-based assay. A high proportion (63%) of the infants were exposed to malaria in utero. Levels of EBV- and tetanus-specific antibodies were similar in malaria-infected mothers and in mothers who had no detectable malaria infection. Malaria-exposed neonates had significantly lower levels of anti-EBNA1, anti-Zta, and anti-EAd antibodies than were seen in their mothers. In utero malaria exposure resulted in significant reductions in transplacental transfer of anti-VCA-p18 and anti-EBNA1 antibodies of 13% and 22%, respectively. Neonates received significantly low levels of anti-Zta and anti-EAd antibodies irrespective of malaria exposure levels. In multivariate analysis, in utero malaria exposure was associated with a significant reduction in the transfer of anti-VCA-p18 and anti-EBNA1 antibodies to the neonates (P = 0.0234 and P = 0.0017, respectively). Malaria during pregnancy results in differential levels of transfer of EBV-specific antibodies from the mother to the fetus. The impaired transplacental transfer of some antibodies may lead to the malaria-exposed neonates being susceptible to early EBV infection.