Carvedilol decreases elevated oxidative stress in human failing myocardium
Carvedilol decreases elevated oxidative stress in human failing myocardium
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DOI：
10.1161/01.cir.0000018605.14470.dd
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发表时间：
2002-06-18
期刊：
影响因子：
37.8
通讯作者：
Ohe, T
中科院分区：
文献类型：
--
作者：
Nakamura, K;Kusano, K;Ohe, T
氧化应激与心力衰竭的发病机制有关。然而，尚未获得人类衰竭心肌中氧化应激产生的直接证据。此外，卡维地洛（一种具有抗氧化活性的血管舒张α-受体阻滞剂）对人类衰竭心脏中氧化应激的影响尚未评估。方法和结果-从23名扩张型心肌病患者和13名心脏功能正常的对照受试者中获得的心肌内膜活检样品进行了4-羟基-2-壬烯醛（HNE）修饰蛋白表达的化学研究，其是主要的脂质过氧化产物。HNE修饰蛋白在所有DCM患者的心肌活检标本中均有表达。在心肌细胞胞浆中表达明显。DCM患者心肌HNE修饰蛋白水平显著高于对照组（P
Background-Oxidative stress has been implicated in the pathogenesis of heart failure. However, direct evidence of oxidative stress generation in the human failing myocardium has not been obtained. Furthermore, the effect of carvedilol, a vasodilating a-blocker with antioxidant activity, on oxidative stress in human failing hearts has not been assessed. This study was therefore designed to determine whether levels of lipid peroxides are elevated in myocardia of patients with dilated cardiomyopathy (DCM) and whether carvedilol reduces the lipid peroxidation level.Methods and Results-Endomyocardial biopsy samples obtained from 23 patients with DCM and 13 control subjects with normal cardiac function were studied immunohistochemically for the expression of 4-hydroxy-2-nonenal (HNE)-modified protein, which is a major lipid peroxidation product. Expression of HNE-modified protein was found in all myocardial biopsy samples from patients with DCM. Expression was distinct in the cytosol of cardiac myocytes. Myocardial HNE-modified protein levels in patients with DCM were significantly increased compared with the levels in control subjects (P