Methanogenic pathways in Methanosphaera stadtmanae.
Methanogenic pathways in Methanosphaera stadtmanae.
复制标题
Methanosphaera stadtmanae 中的产甲烷途径。
DOI：
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发表时间：
1991
影响因子：
2.1
通讯作者：
C. van der Drift
中科院分区：
文献类型：
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作者：
W. M. van de Wijngaard;J. Creemers;G. Vogels;C. van der Drift
甲烷球腔菌（Methanosphaera stadtmanae）将甲醇还原成甲烷的方式与巴氏甲烷八叠球菌（Methanosarcina barkeri）相似。甲醛氧化还原反应的研究表明，甲醛不能将CO2还原为CH 4的主要原因是缺乏活性或存在无活性的CO2还原酶系统和甲基四氢甲烷蝶呤（methyl-H4 MPT）：辅酶M甲基转移酶。未检测到甲烷呋喃，而类似于H4 MPT的蝶呤的存在可以从煮沸提取物中的降解产物中得到证实。
Methanosphaera stadtmanae reduces methanol to CH4 in a similar way as Methanosarcina barkeri. Low activities of 5,10-methylenetetrahydromethanopterin dehydrogenase (MTDH) and reductase (MTR) were found. From studies on formaldehyde oxidation and reduction it was concluded that most likely the inability to reduce CO2 to CH4 was due to the lack of an active or the presence of an inactive CO2 reductase system and methyltetrahydromethanopterin (methyl-H4MPT): coenzyme M methyltransferase. Methanofuran was not detected, while the presence of a pterin, analogous to H4MPT, could be substantiated from its degradation products in boiled extracts.