Phytochemical fingerprint and chemometrics for natural food preparation pattern recognition: an innovative technique in food supplement quality control
Phytochemical fingerprint and chemometrics for natural food preparation pattern recognition: an innovative technique in food supplement quality control
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DOI：
10.1007/s13197-015-2115-6
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发表时间：
2016-02-01
影响因子：
3.1
通讯作者：
Beccaro, G. L.
中科院分区：
文献类型：
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作者：
Donno, D.;Boggia, R.;Beccaro, G. L.
近年来，色谱指纹图谱方法已成为中药和食品质量评价的有效工具之一：由于色谱指纹图谱的复杂性以及色谱仪器和实验条件的不可重复性，化学计量学方法被用来处理色谱指纹图谱。本研究的目的是开发新的分析方法的多元植物化学指纹的生物活性化合物在8个树种芽制剂，常用的植物疗法。方法被用来确定和量化的主要生物活性化合物（多酚，有机酸和维生素），并获得一个特定的植物概况，以评估每个单一的生物活性类的贡献，总芽制备植物复合物。化学计量学方法用于区分不同的基因型，确保植物原料的身份，安全性和质量。所建立的方案是简单的，灵敏度和可靠的，它可以用于评价和质量控制的芽提取物和天然食品补充剂：所提出的方法被成功地应用于商业芽制剂的表征，证明是一个有效的工具，这种植物材料的指纹图谱。本研究开发的新方法为改善具有复杂色谱图的草药材料的分类结果提供了一种很好的选择。应建立“多元色谱指纹图谱”，以便根据其基因型区分草药制剂，避免与其他物种或合成药物的替代、改变或掺杂。
Recently, the fingerprint approach using chromatography has become one of the most effective tools for quality assessment of herbal medicines and food supplements: due to the complexity of the chromatographic fingerprint and the irreproducibility of chromatographic instruments and experimental conditions, chemometric approach is employed to deal with the chromatographic fingerprint. The study was aimed at developing new analytical methods for the multivariate phytochemical fingerprinting of bioactive compounds in eight tree-species bud-preparations, commonly used in phytotherapy. Methods was used to identify and quantify the main bioactive compounds (polyphenols, organic acids and vitamins), and obtain a specific botanical profile in order to assess the contribution of each single bioactive class to the total bud preparation phytocomplex. A chemometric approach was used to distinguish among different genotypes assuring the identity, safety and quality of the botanical raw materials. The established protocol was simple, sensitive and reliable and it could be used for the evaluation and quality control of bud-extracts and natural food supplements: the proposed method was successfully applied to the characterization of commercial bud-preparations, demonstrating to be an effective tool for the fingerprinting of this plant material. The new approach developed in this study represents a good alternative for improving the classification results of herbal materials with complex chromatograms. It should be necessary to develop a "multivariate chromatographic fingerprint", in order to differentiate the herbal preparations according to their genotype, avoiding substitutions, changes or adulterations with other species or synthetic drugs.