Epithelial development in the rat ventral prostate, anterior prostate and seminal vesicle.
Epithelial development in the rat ventral prostate, anterior prostate and seminal vesicle.
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DOI：
10.1159/000147793
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发表时间：
1996
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影响因子：
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通讯作者：
Simon W. Hayward;Laurence S. Baskin;Peter C. Haughney;A. Cunha;B. A. Foster;Rajvir Dahiya;G. Prins;Cunha Gr
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文献类型：
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作者：
Simon W. Hayward;Laurence S. Baskin;Peter C. Haughney;A. Cunha;B. A. Foster;Rajvir Dahiya;G. Prins;Cunha Gr
前列腺和精囊（SV）是男性生殖道的雄激素依赖性分泌腺。这些腺体的上皮细胞产生大量的精液分泌物。本研究的目的是研究细胞角蛋白和雄激素受体（AR）的表达在大鼠SV，前列腺前（AP）和腹侧前列腺（VP）的个体发育。该研究利用器官培养来检查雄激素对这些标记物的发展的影响，并对成年大鼠进行去势来检查雄激素对它们的维持的影响。从妊娠14天到成年，对组织进行检查。SV从一开始就是一个管状器官，而前列腺是由固体上皮索形成的。这些前列腺芽以近端到远端的方式在出生后第1天开始在VP和第5天在AP中形成通道。免疫细胞化学法观察细胞角蛋白和AR的表达。在所有三个腺体中，角蛋白5、7、8、14、18和19最初在所有上皮细胞中均匀表达。在SV中，细胞角蛋白在腔细胞和基底细胞类型之间的分离在出生后4天开始，角蛋白7定位于基底细胞。出生后5天，角蛋白5和14也定位于基底上皮，而角蛋白8和18仅在腔细胞中表达，角蛋白19在整个发育和成年期的所有上皮细胞中表达。在VP和AP的细胞角蛋白分离发生在SV相同的模式。前列腺上皮细胞分化呈近端向远端分化。在近端VP导管角蛋白7和14的基底本地化2天postnatally，而角蛋白5没有明确的分离，直到出生后第9天的基底细胞。在AP角蛋白14是基底本地化的出生后1天，但角蛋白5和7没有共定位到基底细胞，直到第9和12天，分别。孕19天开始，尿生殖窦上皮细胞表达AR。在胚胎发育的19和20天，从AR阳性的尿生殖窦上皮中可见AR阴性的前列腺芽。出生时，在前列腺叶和SV的上皮中均可检测到AR。在无血清器官培养系统中研究了雄激素在前列腺和SV上皮发育中的作用。这些实验表明，前列腺和SV腔和基底上皮细胞类型的分化加速相比，在雄激素的存在下，在体内的情况下，并没有发生在他们的缺席。成年动物去势后，前列腺和SV退化，管腔上皮优先丧失。基底细胞的相对数量增加，但仍观察到一些扁平细胞表达细胞角蛋白的管腔细胞模式。在长期去势动物的前列腺和SV上皮中检测到AR。总之，发现大鼠前列腺来源于未分化的实性上皮索。渠化发生的同时，明确的上皮亚型的分化。上皮AR从出生时开始表达，表达水平随年龄增加而增加。SV从一开始就被疏通，但同样来自未分化的上皮前体。
The prostate and seminal vesicle (SV) are androgen-dependent secretory glands of the male genital tract. The epithelial cells of these glands produce the bulk of the seminal secretions. The objective of the present study was to examine the ontogeny of cytokeratin and androgen receptor (AR) expression in the rat SV, anterior prostate (AP) and ventral prostate (VP). The study utilized organ culture to examine the effects of androgens on the development of these markers and castration of adult rats to examine androgenic effects on their maintenance. Tissues were examined from 14 days of gestation to adulthood. The SV was a tubular organ from its inception while the prostate formed from solid epithelial cords. These prostatic buds canalized in a proximal to distal manner starting at day 1 postnatal in the VP and day 5 in the AP. The expression of cytokeratins and AR was visualized by immunocytochemistry. In all three glands keratins 5, 7, 8, 14, 18 and 19 were initially uniformly expressed in all epithelial cells. In the SV, segregation of cytokeratins between the luminal and basal cell types started at 4 days postnatally with keratin 7 localizing to basal cells. Five days after birth, keratins 5 and 14 were also localized to the basal epithelium, while keratins 8 and 18 were only expressed by luminal cells, Keratin 19 was expressed in all epithelial cells throughout development and into adulthood. In the VP and AP the same pattern of cytokeratin segregation occurred as in the SV. Epithelial differentiation occurred in a proximal to distal fashion in the prostate. In the proximal VP ducts keratins 7 and 14 were basally localized by 2 days postnatally, while keratin 5 did not clearly segregate to basal cells until day 9 after birth. In the AP keratin 14 was basally localized by 1 day postnatal but keratin 5 and 7 did not colocalize to the basal cells until days 9 and 12, respectively. AR were expressed in the epithelium of the urogenital sinus from 19 days of gestation. At 19 and 20 days of embryonic development AR-negative prostatic buds were seen emerging from the AR-positive urogenital sinus epithelium. By birth AR were detectable in the epithelium of both prostatic lobes and the SV. The role of androgens in the development of the prostatic and SV epithelium was investigated in a serum-free organ culture system. These experiments showed that differentiation of prostatic and SV luminal and basal epithelial cell types was accelerated as compared to the in vivo situation in the presence of androgens, and did not occur in their absence. Following castration of adult animals the prostate and SV regressed with preferential loss of luminal epithelium. The relative numbers of basal cells was increased, though some flattened cells expressing a luminal cell pattern of cytokeratins were still observed. AR were detected in the prostatic and SV epithelium of long-term castrated animals. In summary, the rat prostate was found to be derived from undifferentiated solid epithelial cords. Canalization occurred concurrent with the differentiation of clear epithelial subtypes. Epithelial AR were expressed from around the time of birth and expression levels increased with age. The SV was canalized from its inception but likewise was derived from an undifferentiated epithelial precursor.