Characteristics of maltose transporter activity in an ale and lager strain of the yeast Saccharomyces cerevisiae
Characteristics of maltose transporter activity in an ale and lager strain of the yeast Saccharomyces cerevisiae
复制标题
DOI：
10.1111/j.1472-765x.1996.tb01356.x
复制
发表时间：
1996-12-01
影响因子：
2.4
通讯作者：
Stewart, GG
中科院分区：
文献类型：
--
作者：
Crumplen, RM;Slaughter, JC;Stewart, GG
对艾尔啤酒和啤酒吸收麦芽糖的动力学研究表明，与实验室酵母相比，K-m 值更低，V-max 值更高。 Eadie-Hofstee 图是双相的，并且巯基试剂的抑制与特定转运蛋白而非非特定吸附的低亲和力摄取一致。两种菌株的高亲和力麦芽糖转运均受到半乳糖、CCCP、2,4-DNP 和 NEM 的抑制。相比之下，葡萄糖和 pCMBS 抑制艾尔酵母的高亲和力转运，但不抑制啤酒酵母的高亲和力转运。就 pCMBS 而言，差异可能是由于麦芽糖转运蛋白中单个氨基酸的变化造成的。
Kinetic studies on maltose uptake by ale and lager showed lower K-m and higher V-max values than for laboratory yeasts. Eadie-Hofstee plots were biphasis and inhibition by sulphydryl reagents was consistent with low affinity uptake by a specific transporter rather than by non-specific adsorption. High affinity maltose transport by both strains was inhibited by galactose, CCCP, 2,4-DNP and NEM. In contrast, glucose and pCMBS inhibited high affinity transport by the ale yeast but not by the lager yeast. In the case of pCMBS the difference could result form a single amino acid change in the maltose transporter.