Ultraviolet native fluorescence detection in capillary electrophoresis using a metal vapor NeCu laser.
Ultraviolet native fluorescence detection in capillary electrophoresis using a metal vapor NeCu laser.
复制标题
使用金属蒸气 NeCu 激光器进行毛细管电泳中的紫外天然荧光检测。
DOI：
10.1021/ac010458z
复制
发表时间：
2001
影响因子：
7.4
通讯作者：
Sweedler,JV
中科院分区：
文献类型：
--
作者：
Zhang,X;Sweedler,JV
在毛细管电泳（CE）中，紫外（UV）激发激光诱导天然荧光（LINF）检测在测定含有酪氨酸或色氨酸残基的肽、儿茶酚胺、吲哚胺和许多其他类别的被分析物时提供了令人印象深刻的性能指标，当被UV辐射激发时具有可观的荧光。CE-LINF系统中天然荧光检测方案的最大缺点之一是在200 - 300 nm的深紫外波长范围内激发所需的激光器的费用和复杂性。一种改进的“交钥匙”NeCu激光器在248.6 nm的界面鞘流为基础的CE系统获得的性能类似的大帧倍频氩离子激光器。5-羟色胺和多巴胺的检测限（27 nM和8 μM，分别为100-nL注射液）与使用257 nm倍频Ar离子激光获得的检测限（21 nM和8 μM，分别）相似。从单细胞电泳图中检测到的阿托宁相关分析物的一个例子证明了这种系统用于质量限制测量的性能。
Ultraviolet (UV) excitation for laser-induced native fluorescence (LINF) detection in capillary electrophoresis (CE) offers impressive performance figures of merit when assaying peptides containing tyrosine or tryptophan residues, catecholamines, indolamines, and a number of other classes of analytes with appreciable fluorescence when excited by UV radiation. One of the largest drawbacks of native fluorescence detection schemes in CE-LINF systems has been the expense and the complexity of the lasers required for excitation in the deep UV wavelength range of 200−300 nm. An improved “turnkey” NeCu laser operating at 248.6 nm interfaced to a sheath flow-based CE system obtains a performance similar to that of large frame frequency-doubled Ar ion lasers. The detection limits for serotonin and dopamine (27 nM and 8 μM, respectively, for ∼3-nL injection) are similar to those obtained using a frequency-doubled Ar ion laser at 257 nm (21 nM and 8 μM, respectively). An example of the detection of serotonin-related analytes from a single-cell electropherogram demonstrates the performance of such a system for mass-limited measurements.