High-resolution chemical imaging of labile phosphorus in the rhizosphere of Brassica napus L. cultivars
High-resolution chemical imaging of labile phosphorus in the rhizosphere of Brassica napus L. cultivars
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DOI：
10.1016/j.envexpbot.2011.11.026
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发表时间：
2012-04-01
影响因子：
5.7
通讯作者：
Wenzel, Walter W.
中科院分区：
文献类型：
--
作者：
Santner, Jakob;Zhang, Hao;Wenzel, Walter W.
磷是植物生长和作物生产的主要限制因子。磷的溶解，吸收植物根系和流出导致复杂的，动态循环的P在植物根系附近。然而，在相关空间尺度（亚毫米）上直接观察根际磷动态仍然缺乏。采用薄膜扩散梯度（DGT）结合激光烧蚀电感耦合等离子体质谱（LA-ICP-MS）技术，对油菜根周围溶解态磷浓度进行了化学成像。数学模拟用于研究化学成像技术的能力。我们在这里首次展示了沿着根轴和根尖的局部磷释放。沿着根轴和根尖的局部磷释放。我们的研究结果指出，在差异磷吸收效率的两个调查B。油菜品种本研究推进了目前对根际磷素动态的认识，从而对植物磷素营养的认识。该技术可用于解开基因型效应对根际磷动员，从而有助于培育磷高效作物品种。(C)2011 Elsevier B. V.保留所有权利。
Phosphorus is a major limiting factor in plant growth and crop production. Phosphorus solubilisation, uptake by plant roots and efflux lead to complex, dynamic cycling of P in the vicinity of plant roots. However, direct observation of P dynamics in the rhizosphere at relevant spatial scales (sub-mm) is still lacking. Chemical imaging of the dissolved P concentration around Brassica napus roots was accomplished using diffusive gradients in thin films (DGT) coupled with laser-ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Mathematical simulations served for investigating the capabilities of the chemical imaging technique. We show here, for the first time, localised P release along root axes and at root apices. Our results point at differential P uptake efficiencies of the two investigated B. napus cultivars. This study advances the current understanding of P dynamics in the rhizosphere and thus of plant P nutrition. This technique can serve to unravel the genotypic effects on rhizosphere mobilisation of P and hence assist breeding of highly P efficient crop cultivars. (C) 2011 Elsevier B.V. All rights reserved.