Vitamin B1 functions as an activator of plant disease resistance
Vitamin B1 functions as an activator of plant disease resistance
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DOI：
10.1104/pp.104.058693
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发表时间：
2005-07-01
期刊：
影响因子：
7.4
通讯作者：
Lee, YH
中科院分区：
文献类型：
--
作者：
Ahn, IP;Kim, S;Lee, YH
维生素B 1（硫胺素）是人体必需的营养素。维生素B 1缺乏会导致脚气病，它会扰乱中枢神经和循环系统。在以稻米为主要食物的国家，硫胺素缺乏症很普遍，因为稻米的抛光去除了谷物中的大部分硫胺素。我们在这里证明，硫胺素，除了它的营养价值，诱导植物的系统获得性抗性（SAR）。硫胺素处理的水稻、拟南芥和蔬菜作物表现出对真菌、细菌和病毒感染的抗性。硫胺素处理诱导水稻和其它植物中致病相关（PR）基因的瞬时表达。此外，硫胺素处理增强更强和更快的PR基因表达和蛋白激酶C活性的上调。硫胺素对抗病性和防御相关基因表达的影响在整个植株中系统动员，并持续15 d以上。用硫胺素处理拟南芥生态型哥伦比亚-0植物导致PR-1的激活，但不导致PDF1.2的激活。此外，硫胺素防止细菌感染的拟南芥突变体不敏感的茉莉酸或乙烯，但不是在突变体受损的SAR转导途径。这些结果清楚地表明，硫胺素诱导SAR在植物中通过水杨酸和Ca 2+相关的信号通路。这些发现为开发控制植物病害的替代策略提供了新的范式。
Vitamin B 1 (thiamine) is an essential nutrient for humans. Vitamin B 1 deficiency causes beriberi, which disturbs the central nervous and circulatory systems. In countries in which rice (Oryza sativa) is a major food, thiamine deficiency is prevalent because polishing of rice removes most of the thiamine in the grain. We demonstrate here that thiamine, in addition to its nutritional value, induces systemic acquired resistance (SAR) in plants. Thiamine- treated rice, Arabidopsis (Arabidopsis thaliana), and vegetable crop plants showed resistance to fungal, bacterial, and viral infections. Thiamine treatment induces the transient expression of pathogenesis- related (PR) genes in rice and other plants. In addition, thiamine treatment potentiates stronger and more rapid PR gene expression and the up- regulation of protein kinase C activity. The effects of thiamine on disease resistance and defense-related gene expression mobilize systemically throughout the plant and last for more than 15 d after treatment. Treatment of Arabidopsis ecotype Columbia-0 plants with thiamine resulted in the activation of PR-1 but not PDF1.2. Furthermore, thiamine prevented bacterial infection in Arabidopsis mutants insensitive to jasmonic acid or ethylene but not in mutants impaired in the SAR transduction pathway. These results clearly demonstrate that thiamine induces SAR in plants through the salicylic acid and Ca2+- related signaling pathways. The findings provide a novel paradigm for developing alternative strategies for the control of plant diseases.