Vagal mechanoreceptors and chemoreceptors in mouse stomach and esophagus
Vagal mechanoreceptors and chemoreceptors in mouse stomach and esophagus
复制标题
DOI：
10.1152/jn.00785.2001
复制
发表时间：
2002-04-01
影响因子：
2.5
通讯作者：
Blackshaw, LA
中科院分区：
文献类型：
--
作者：
Page, AJ;Martin, CM;Blackshaw, LA
我们使用一种新的体外小鼠迷走神经-胃-食道标本来研究外周迷走神经传入末梢的特性。我们发现了两种类型的机械感受性纤维，即粘膜受体和张力受体。它们的区别在于它们对冯·弗雷毛发的粘膜抚触和通过爪子-悬臂系统施加的圆形张力敏感。与在类似的雪貂组织中发现的胃-食道传入神经进行了比较。小鼠张力受体对环状张力的反应明显大于雪貂张力和张力/粘膜受体。类似地，小鼠粘膜受体对粘膜抚触的反应显着大于雪貂和张力/粘膜受体。47%的小鼠粘膜受体和50%的张力受体对施加到感受野的一种或多种药物或化学刺激有反应。它们包括α，β-亚甲基三磷酸腺苷(10(-6)至10(-3)M)、5-羟色胺(10(-6)至10(-3)M)和盐酸(10(-2)至10(-1)M)。药物反应呈浓度依赖性。100%的粘膜受体和61%的张力受体对胆汁(1：8~1：1稀释)有反应。第三种类型的纤维被胆汁吸收。在胆汁暴露之前，这些纤维机械上不敏感，而且没有声音。总之，我们在小鼠中发现了三种类型的胃-食道迷走神经传入纤维：粘膜机械感受器、张力感受器和胆汁激活的特异性化学感受器。
We used a novel in vitro mouse vagus-gastro-esophageal preparation to study the properties of peripheral vagal afferent endings. We found two types of mechanoreceptive fiber, mucosal receptors and tension receptors. These were distinguished by their sensitivity to mucosal stroking with von Frey hairs and circular tension applied via a claw-cantilever system. A comparison was made with gastro-esophageal afferents found in a similar preparation of ferret tissue. Responses of mouse tension receptors to circular tension were significantly greater than ferret tension and tension/mucosal receptors. Similarly the responses of mouse mucosal receptors to mucosal stroking were significantly greater than ferret mucosal and tension/mucosal receptors. Forty-seven percent of mouse mucosal receptors and 50% of tension receptors responded to one or more drugs or chemical stimuli applied to the receptive field. These included alpha,beta-methylene ATP (10(-6) to 10(-3) M), 5-hydroxytryptamine (10(-6) to 10(-3) M), and hydrochloric acid (10(-2) to 10(-1) M). Drug responses were concentration dependent. One hundred percent of mucosal receptors and 61% of tension receptors tested responded to bile (1:8 to 1:1 dilution). A third type of fiber was recruited by bile. These fibers were mechanically insensitive and silent prior to bile exposure. In conclusion, we have shown three types of gastro-esophageal vagal afferent fibers in the mouse: mucosal mechanoreceptors, tension receptors, and specific chemoreceptors activated by bile.