Preservation of metabolic reserves and function after storage of myocytes in hypothermic UW solution
Preservation of metabolic reserves and function after storage of myocytes in hypothermic UW solution
复制标题
DOI：
10.1152/ajpcell.2001.281.3.c758
复制
发表时间：
2001-09-01
影响因子：
5.5
通讯作者：
Haworth, RA
中科院分区：
文献类型：
--
作者：
Hegge, JO;Southard, JH;Haworth, RA
分离的大鼠肌细胞在威斯康星大学溶液中厌氧冷藏长达 24 小时，损失了 95% 的 ATP 和 100% 的糖原。当在含有 Ca 的 Krebg-Henseleit (KH) 培养基中重新加热时，它们会发生挛缩，除非延迟添加 Ca。在后一种情况下，通过刺激诱导的细胞缩短来测量的细胞功能令人惊讶地得到了很好的保留。需氧储存的细胞在复温时对 Ca 具有抵抗力，但仍损失了 96% 的糖原以及 46% 的 ATP。在 pH 6.2 下与底物葡萄糖和丙酮酸有氧孵育 48 小时的细胞保留了 77% 的 ATP 和 59% 的糖原，具有良好的细胞形态。 pH 6.2 时，对 ATP 的需求仅为 pH 7.4 时的 55%。然而，复温后，这些细胞的功能并不比厌氧储存的细胞更好，尽管它们对异丙肾上腺素的正性肌力反应有所改善。我们得出的结论是：1）低 pH 底物的有氧条件可以很好地保存心肌细胞的代谢储备 48 小时，部分原因是减少了对 ATP 的需求； 2) 复温条件对于代谢储存严重耗尽的厌氧储存细胞至关重要； 3) 令人惊讶的是，卸载的细胞功能对严重代谢剥夺时期不敏感。
Isolated rat myocytes cold stored anaerobically up to 24 h in University of Wisconsin solution lost 95% of their ATP and 100% of their glycogen. They underwent contracture when rewarmed mi a Krebg-Henseleit (KH) medium that contained Ca unless Ca addition was delayed. In the latter case, cell function, measured by stimulation-induced cell shortening, was surprisingly well retained. Aerobically stored cells were resistant to Ca on rewarming, although 96% of glycogen was still lost, along with 46% of ATP. Cells that were incubated for 48 h aerobically with the substrates glucose and pyruvate at pH 6.2 retained 77% of their ATP and 59% of their glycogen, with good cell morphology. At pH 6.2, the demand for ATP was only 55% of that at pH 7.4. However, after rewarming, these cells functioned no better than anaerobically stored cells, although their inotropic response to isoproterenol was improved. We conclude that 1) aerobic conditions with substrates at low pH preserve myocyte metabolic reserves well for 48 h, partly by reducing the demand for ATP; 2) rewarming conditions are critical for anaerobically stored cells with metabolic stores that are severely depleted; and 3) unloaded cell function is surprisingly insensitive to a period of severe metabolic deprivation.