Long non-coding RNA profiling of human lymphoid progenitor cells reveals transcriptional divergence of B cell and T cell lineages.
Long non-coding RNA profiling of human lymphoid progenitor cells reveals transcriptional divergence of B cell and T cell lineages.
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DOI：
10.1038/ni.3299
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发表时间：
2015-12
影响因子：
30.5
通讯作者：
Crooks GM
中科院分区：
文献类型：
--
作者：
Casero D;Sandoval S;Seet CS;Scholes J;Zhu Y;Ha VL;Luong A;Parekh C;Crooks GM
为了阐明在出生后生活中调节人类淋巴定型的转录景观，我们使用RNA测序来组装跨越B和T淋巴特化的最早阶段的人类骨髓和胸腺祖细胞的长非编码转录组。通过对这些罕见群体的分析，发现了3000多个新的长非编码RNA基因（lncRNA）。类胚定型的特点是lncRNA的表达模式是高度阶段特异性和谱系特异性比蛋白质编码模式。与邻近lncRNA基因共表达的蛋白质编码基因富集了与淋巴分化相关的本体。全球lncRNA表达模式的精致细胞类型特异性独立地揭示了人类骨髓和胸腺中最早的祖细胞之间新的发育关系。
To elucidate the transcriptional landscape that regulates human lymphoid commitment during postnatal life, we used RNA sequencing to assemble the long non-coding transcriptome across human bone marrow and thymic progenitors spanning the earliest stages of B and T lymphoid specification. Over 3000 novel long non-coding RNA genes (lncRNAs) were revealed through the analysis of these rare populations. Lymphoid commitment was characterized by lncRNA expression patterns that were highly stage-specific and more lineage-specific than protein coding patterns. Protein-coding genes co-expressed with neighboring lncRNA genes were enriched for ontologies related to lymphoid differentiation. The exquisite cell-type specificity of global lncRNA expression patterns independently revealed new developmental relationships between the earliest progenitors in the human bone marrow and thymus.