Genetic analysis of pbp2x in clinical Streptococcus pneumoniae isolates in Quebec, Canada
Genetic analysis of pbp2x in clinical Streptococcus pneumoniae isolates in Quebec, Canada
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DOI：
10.1093/jac/dki118
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发表时间：
2005-06-01
影响因子：
5.2
通讯作者：
Roger, M
中科院分区：
文献类型：
--
作者：
Granger, D;Boily-Larouche, G;Roger, M
目的：研究在加拿大魁北克省耐青霉素肺炎链球菌分离株的青霉素结合蛋白（131313）2x中发现的氨基酸基序的性质，并获得关于这些改变的流行率的初步信息。方法：pbp 2x基因组区域包含密码子178-703，其中包括转肽酶结构域的整个区域，结果：PBP 2x内的多样性程度与β-内酰胺类抗生素耐药性的增加有关。与对照青霉素敏感菌株R6相比，PSSP中平均有5.0 +/- 1.8个突变，PISP中平均有37.9 +/- 4.4个突变，PRSP分离株中平均有63.0 +/- 2.0个突变。在魁北克分离的PISP菌株中鉴定出至少六种不同的氨基酸谱。相比之下，所有的PRSP分离株共享一个类似的模式，改变的氨基酸序列相比，从易感strains.Conclusions：这些数据将是有用的，在未来的研究中，以监测与P-内酰胺耐药性的出现和传播在魁北克的遗传变化。
Objectives: To investigate the nature of the amino acid motifs found in penicillin-binding protein (131313) 2x of penicillin-resistant Streptococcus pneumoniae isolates across the province of Quebec (Canada), and to obtain preliminary information regarding the prevalence of these alterations.Methods: The pbp2x genomic region encompassing codons 178-703, which includes the entire region of the transpeptidase domain, was sequenced and compared for 52 clinical isolates comprising 20 penicillin-susceptible (PSSP), 20 penicillin-intermediate (PISP) and 12 penicillin-resistant (PRSP) pneumococci.Results: The degree of diversity within PBP2x correlated with increased resistance to beta-lactam antibiotics. There were an average of 5.0 +/- 1.8 mutations in PSSP, 37.9 +/- 4.4 in PISP, and 63.0 +/- 2.0 in PRSP isolates when compared with the control penicillin-susceptible strain R6. At least six distinct amino acid profiles were identified among PISP strains isolated in Quebec. In contrast, all PRSP isolates shared a similar pattern of altered amino acids compared with the sequence from susceptible strains.Conclusions: These data will be useful in future studies to monitor the genetic changes associated with the emergence and spread of P-lactam resistance in Quebec.