Expression Analysis of Prestin and Selected Transcription Factors in Newborn Rats
Expression Analysis of Prestin and Selected Transcription Factors in Newborn Rats
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DOI：
10.1007/s10571-011-9708-z
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发表时间：
2011-10-01
影响因子：
4
通讯作者：
Mazurek, Birgit
中科院分区：
文献类型：
--
作者：
Gross, Johann;Angerstein, Maximilian;Mazurek, Birgit
转录因子在基因表达调控中起着核心作用。为了分析选定的转录因子与外毛细胞运动蛋白prestin的共表达模式，我们应用实时荧光定量聚合酶链式反应方法结合了几种信息：(I)出生后发育期间的表达变化，(Ii)Corti器官器官培养物暴露于显著影响prestin表达的因素[甲状腺激素(T4)、维甲酸(RA)、丁酸(BA)、KCl浓度升高]以及(Iii)沿顶端-基础梯度的变化。我们发现，POU家族成员Tf BRN-3c(Pou4f3)的mRNA水平与出生后发育过程中以及在添加T4(0.5 mM)、BA(0.5-2.0 mM)和高浓度KCl(50 MM)的培养中对prestin的调节显著相关。天然活性的TFC/EBPB(CCAAT/增强子结合蛋白β)的mRNA水平与出生后发育过程中以及暴露于T4和RA(50-100 mM)的培养物中prestin的表达呈正相关。在T4和高KCl浓度的培养中，钙依赖的TFCARF的mRNA水平与prestin的表达显著相关。观察到的共表达模式可能表明，在所研究的条件下，转录因子BRN-3c、C/EBPB和CARF有助于调节prestin的表达。
Transcription factors (TFs) have a central role to play in regulating gene expression. To analyze the co-expression patterns of selected TFs with the motor protein prestin of the outer hair cells, we applied an real-time PCR approach combining several kinds of information: (i) expression changes during postnatal development, (ii) expression changes by exposure of organotypic cultures of the organ of Corti to factors which significantly affect prestin expression [thyroid hormone (T4), retinoic acid (RA), butyric acid (BA), increased KCl concentration] and (iii) changes along the apical-basal gradient. We found that the mRNA levels of the TF Brn-3c (Pou4f3), a member of the POU family, are significantly associated with the regulation of prestin during postnatal development and in cultures supplemented with T4 (0.5 mu M), BA (0.5-2.0 mM), and high KCl (50 mM) concentration. The mRNA level of the constitutively active TF C/ebpb (CCAAT/enhancer binding protein beta) correlates positively with the prestin expression during postnatal development and in cultures exposed to T4 and RA (50-100 mu M). The mRNA levels of the calcium-dependent TF CaRF correlates significantly with the prestin expression in cultures exposed to T4 and high KCl concentration. The observed coexpression patterns may suggest that the TFs Brn-3c, C/ebpb, and Carf contribute to regulating the expression of prestin under the investigated conditions.