Three luteinizing hormone-releasing hormone like substances in a teleost fish brain: none identical with the mammalian LH-RH decapeptide.
Three luteinizing hormone-releasing hormone like substances in a teleost fish brain: none identical with the mammalian LH-RH decapeptide.
复制标题
硬骨鱼大脑中存在三种类似黄体生成素释放激素的物质：与哺乳动物的 LH-RH 十肽均不相同。
DOI：
10.1016/s0006-291x(82)80032-6
复制
发表时间：
1982
影响因子：
3.1
通讯作者：
Jackson,IM
中科院分区：
文献类型：
--
作者：
Barnett,FH;Sohn,J;Reichlin,S;Jackson,IM
鳕鱼脑的醋酸提取物含有三种不同的免疫反应性(IR)促黄体激素释放激素(LH-RH)样物质，它们在层析分离(分子筛分、离子交换和高效液相色谱)上的行为与哺乳动物的促黄体激素释放激素十肽不同，并且它们与两种针对LH-RH分子不同区域的抗血清发生反应。其中一种类似IR的LH-RH物质是一种较大的分子量化合物(1800~4000道尔顿)，它可能是在N-末端延伸的LH-RH的前体。另外两个与哺乳动物的促黄体激素释放激素的大小大致相同，它们的性质可以通过在十肽的C-末端区域发生单一氨基酸替换来最好地解释。
Acetic acid extracts of codfish brain contain three different immunoreactive (IR) luteinizing hormone-releasing hormone (LH-RH) like materials which differ from the mammalian LH-RH decapeptide by their behavior on chromatographic separation (molecular sieving, ion exchange and high performance liquid chromatography), and their reaction with two well characterized antisera directed against different regions of the LH-RH molecule. One of the IR LH-RH like substances is a larger molecular weight compound (1800 to 4,000 daltons), which may be a precursor of LH-RH extended at the N-terminus. The other two are approximately the same size as mammalian LH-RH and their nature is best explained by the occurrence of single amino acid substitutions at the C-terminal region of the ‘authentic’ decapeptide.