IRON-DEXTRAN ANTIBODY CONJUGATES - GENERAL-METHOD FOR SIMULTANEOUS STAINING OF 2 COMPONENTS IN HIGH-RESOLUTION IMMUNOELECTRON MICROSCOPY
IRON-DEXTRAN ANTIBODY CONJUGATES - GENERAL-METHOD FOR SIMULTANEOUS STAINING OF 2 COMPONENTS IN HIGH-RESOLUTION IMMUNOELECTRON MICROSCOPY
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DOI：
10.1073/pnas.76.7.3392
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
SINGER, SJ
中科院分区：
文献类型：
--
作者：
DUTTON, AH;TOKUYASU, KT;SINGER, SJ
本文报道了一种由铁-葡聚糖（Imposil）颗粒共价键合到抗体分子上的电子致密抗体偶联物的制备和性质。Imposil-抗体缀合物的透射电子显微镜实验证明了它们作为高分辨率特异性免疫染色剂的适用性，特别是对于与铁蛋白-抗体缀合物结合的同时双染色实验。
The preparation and properties of an electron-dense antibody conjugate, made by the covalent bonding of an iron-dextran (Imposil) particle to an antibody molecule were reported. Transmission electron microscopic experiments with the Imposil-antibody conjugates demonstrated their suitability as specific immunostains at high resolution, particularly for simultaneous double staining experiments in conjunction with ferritin-antibody conjugates.