SEQUENCE STRUCTURES OF A MOUSE MAJOR URINARY PROTEIN GENE AND PSEUDOGENE COMPARED
SEQUENCE STRUCTURES OF A MOUSE MAJOR URINARY PROTEIN GENE AND PSEUDOGENE COMPARED
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DOI：
10.1002/j.1460-2075.1985.tb04059.x
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发表时间：
1985-01-01
期刊：
影响因子：
11.4
通讯作者：
BISHOP, JO
中科院分区：
文献类型：
--
作者：
CLARK, AJ;GHAZAL, P;BISHOP, JO
实验室小鼠品系携带.apprx病毒。每个单倍体基因组中有35个主要尿蛋白(MUP)基因，紧密聚集在4号染色体上。大多数属于两个主要组(组1和组2)。现有证据有力地表明，组1基因是活性的，而组2基因是假基因。在这里，我们给出了一个组1基因和一个组2基因的完整序列和700bp的侧翼序列。第一组基因的序列与其活性一致。第二组基因在第一组基因所定义的阅读框中包含两个终止密码子和一个移码突变，编码的信号肽是25个，而不是19个氨基酸。第2组基因与第1组基因在其他方面不同：TATA盒上游和内含子3的缺失、TATA盒本身的碱基改变、内含子6的剪接受体边界上的2bp重复、改变的聚(A)添加信号和起始密码子的1碱基缺失5‘’。其中一些差异可能解释了小鼠肝脏中第一组mRNA水平高出10到20倍，以及第一组和第二组转录本主要是不同剪接的事实。终止密码子的存在意味着在第一组基因的背景下，第二组基因是假基因。然而，有一些证据表明，它将编码的成熟六肽可能具有生物活性。这两个基因的12个受体剪接点都在外显子边界含有相同的序列ACAG。因此，该区域与剪接供体位点显示出异常高的碱基配对同源性。在每个内含子的受体位置边界的17~35bp5‘’之间，发现了一个与CTGAC序列具有中等到高度同源性的序列。
Laboratory mouse strains carry .apprx. 35 major urinary protein (MUP) genes per haploid genome, tightly clustered together on chromosome 4. Most belong to two main groups (Groups 1 and 2). The available evidence strongly suggests that the Group 1 genes are active while Group 2 genes are pseudogenes. Here we present the complete sequence of a Group 1 gene and a Group 2 gene and 700 bp of flanking sequence. The sequence of the Group 1 gene is consistent with its being active. The Group 2 gene contains two stop codons and a frame-shift mutation in the reading frame defined by the Group 1 gene, and would code for a signal peptide 25 rather than 19 amino acids long. The Group 2 gene differs from the Group 1 gene in other ways: as deletion upstream of the TATA box and another in intron 3, a base change in the TATA box itself, a 2 bp duplication at the splice acceptor boundary of intron 6, an altered poly(A) addition signal and a 1-base deletion 5'' to the initiation condon. Some of these differences may explain the 10- to 20-fold higher level of Group 1 mRNA in mouse liver, and the fact that Group 1 and Group 2 transcripts are mainly spliced differently. The presence of the stop condon means that the Group 2 gene is a pseudogene in the context of the Group 1 gene. However, there is some evidence that the mature hexapeptide that it would code for may have biological activity. The 12 acceptor splices sites of the two genes all contain the identical sequence ACAG at the exon boundary. As a result this region shows an unusally high level of base-pairing homology with the splice donor site. A sequence showing a moderate to high homology with the sequence CTGAC is found between 17 and 35 bp 5'' to the acceptor site boundary in every intron.