LOCATION OF THE GENE (NDK) FOR NUCLEOSIDE DIPHOSPHATE KINASE ON THE PHYSICAL MAP OF THE ESCHERICHIA-COLI CHROMOSOME
LOCATION OF THE GENE (NDK) FOR NUCLEOSIDE DIPHOSPHATE KINASE ON THE PHYSICAL MAP OF THE ESCHERICHIA-COLI CHROMOSOME
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DOI：
10.1128/jb.173.11.3276.1991
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发表时间：
1991-06-01
影响因子：
3.2
通讯作者：
INOUYE, M
中科院分区：
文献类型：
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作者：
HAMA, H;LERNER, C;INOUYE, M
核苷二磷酸激酶对于将所有核苷三磷酸和脱氧核苷三磷酸的细胞浓度维持在功能水平至关重要 (3)。来自黄色粘球菌的这种酶的基因 (ndk) 已被克隆，并且其 DNA 序列也已确定 (8, 9)。遗传实验表明 ndk 基因对于 M. xanthus 细胞生长至关重要 (8, 9)。最近，来自各种真核生物（盘基网柄菌 [7]、大鼠 [5]、小鼠 [12]、人类 [12] 和果蝇 [2]）的 ndk 基因也已被克隆和测序。这些来自不同来源的核苷二磷酸激酶显示出显着的序列相似性 (2)。根据该共有序列，合成寡核苷酸引物。基于 ndk 基因位于鼠伤寒沙门氏菌 (11) 的 hisS 附近这一事实，来自 8E3（噬菌体 427）、2D5 gua 的噬菌体 DNA
Nucleoside diphosphate kinase is essential to maintain the cellular concentrations of all nucleoside triphosphates and deoxynucleoside triphosphates at functional levels (3). The gene (ndk) for this enzyme from Myxococcus xanthus has been cloned, and its DNA sequence has been determined (8, 9). A genetic experiment indicated that the ndk gene is essential for cell growth of M. xanthus (8, 9). Recently the ndk genes from various eukaryotes (Dictyostelium discoi-deum [7], rat [5], mouse [12], human [12], and Drosophila [2]) have also been cloned and sequenced. These nucleoside diphosphate kinases from different sources show substantial sequence similarities (2). From this consensus sequence, oligonucleotide primers were synthesized. On the basis of the fact that the ndk gene is located near hisS in Salmonella typhimurium (11), phage DNAs from 8E3 (phage 427), 2D5 gua