Genome organization in dicots:: Genome duplication in Arabidopsis and synteny between soybean and Arabidopsis
Genome organization in dicots:: Genome duplication in Arabidopsis and synteny between soybean and Arabidopsis
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DOI：
10.1073/pnas.070430597
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发表时间：
2000-04-11
影响因子：
11.1
通讯作者：
Shoemaker, RC
中科院分区：
文献类型：
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作者：
Grant, D;Cregan, P;Shoemaker, RC
利用定位到大豆连锁群AZ、J和L的DNA序列的概念翻译，研究了大豆和拟南芥之间的共性。在这些连锁群和所有四条拟南芥染色体之间发现了共性，其中GenBank包含了足够的序列来确定共性。大豆连锁群AZ(SoyA2)和拟南芥I染色体几乎在其全长上表现出显著的共性，只需要2-3条染色体重排就能使图谱基本一致。在SoyA2和拟南芥的IV和V染色体之间(靠近RPP5和RPP8基因)以及大豆A2和拟南芥的I和V染色体之间(靠近PhyA和PhyC基因)发现了较小的同源片段。这些亚染色体共线区域本身是同源的，这表明拟南芥在进化过程中经历了多次节段性复制或可能是完全基因组复制。大豆同源连锁群J和L与拟南芥染色体II和IV的同源性也揭示了拟南芥存在节段性复制的证据。进一步支持这一假说的是，在拟南芥中观察到大豆Vsp27和Bng181的同源物(三个位置)与大豆A256的紫色酸性磷酸酶类序列和同源物(五个位置)的非常紧密的连锁。模拟表明，我们报告的共性和重复不太可能是在我们分析同源报告的过程中偶然出现的。
Synteny between soybean and Arabidopsis was studied by using conceptual translations of DNA sequences from loci that map to soybean linkage groups AZ, J, and L. Synteny was found between these linkage groups and all four of the Arabidopsis chromosomes, where GenBank contained enough sequence for synteny to be identified confidently. Soybean linkage group AZ (soyA2) and Arabidopsis chromosome I showed significant synteny over almost their entire lengths, with only 2-3 chromosomal rearrangements required to bring the maps into substantial agreement. Smaller blocks of synteny were identified between soyA2 and Arabidopsis chromosomes IV and V (near the RPP5 and RPP8 genes) and between soyA2 and Arabidopsis chromosomes I and V (near the PhyA and PhyC genes). These subchromosomal syntenic regions were themselves homeologous, suggesting that Arabidopsis has undergone a number of segmental duplications or possibly a complete genome duplication during its evolution. Homologies between the homeologous soybean linkage groups J and L and Arabidopsis chromosomes II and IV also revealed evidence of segmental duplication in Arabidopsis. Further support for this hypothesis was provided by the observation of very close linkage in Arabidopsis of homologs of soybean Vsp27 and Bng181 (three locations) and purple acid phosphatase-like sequences and homologs of soybean A256 (five locations). Simulations show that the synteny and duplications we report are unlikely to have arisen by chance during our analysis of the homology reports.