Spatial patterns of Aquificales in deep-sea vents along the Eastern Lau Spreading Center (SW Pacific)
Spatial patterns of Aquificales in deep-sea vents along the Eastern Lau Spreading Center (SW Pacific)
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DOI：
10.1016/j.syapm.2014.04.002
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发表时间：
2014-09-01
影响因子：
3.4
通讯作者：
Reysenbach, Anna-Louise
中科院分区：
文献类型：
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作者：
Ferrera, Isabel;Banta, Amy B.;Reysenbach, Anna-Louise
与活跃喷发的深海热液矿床有关的微生物多样性与热液流体的地球化学密切相关。虽然这些矿床的主要成员驱动的微生物群落的结构，它是不太好理解的丰度较低的群体是否密切相关的地球化学环境，或驱动可能由生物因素，如微生物群落的相互作用。我们使用的自然地球化学梯度，存在于弧后盆地，东劳扩张中心和Valu-Fa海岭（ELSC/VFR）在西南太平洋，探讨是否化能无机营养Aquificales的地理位置，主岩的喷口场或存款类型的影响。采用克隆、DNA指纹（DGGE）和富集培养方法相结合的方法，检测到以前在海洋喷口描述的该目所有属，即，脱硫杆菌属、嗜热菌属、Aquilex、Hydrogenivirga、Persephonella和Hydrogenothermus。克隆文库和DGGE之间的比较显示不同Aquificales的分布模式相似，而检索的富集培养物的结果不同。然而，使用的培养为基础的和独立的方法提供了互补的系统发育多样性的Aquificales在这些系统中的概述。总之，这项调查显示，ELSC/VFR包含一些最大的Aquificales多样性以往任何时候都在深海喷口地区的报告，多样性模式是联系在一起的地理和地球化学的系统，这个地球化学多样的弧后盆地可能窝藏Aquificales的新成员。(C)2014 Elsevier GmbH. All rights reserved.
The microbial diversity associated with actively venting deep-sea hydrothermal deposits is tightly connected to the geochemistry of the hydrothermal fluids. Although the dominant members of these deposits drive the structure of the microbial communities, it is less well understood whether the lower abundance groups are as closely connected to the geochemical milieu, or driven perhaps by biotic factors such as microbial community interactions. We used the natural geochemical gradients that exist in the back-arc basin, Eastern Lau Spreading Center and Valu-Fa Ridge (ELSC/VFR) in the Southwestern Pacific, to explore whether the chemolithotrophic Aquificales are influenced by geographical location, host-rock of the vent field or deposit type. Using a combination of cloning, DNA fingerprinting (DGGE) and enrichment culturing approaches, all genera of this order previously described at marine vents were detected, i.e., Desulfurobacterium, Thermovibrio,Aquilex, Hydrogenivirga, Persephonella and Hydrogenothermus. The comparison between clone libraries and DGGE showed similar patterns of distribution of different Aquificales whereas results differed for the enrichment cultures that were retrieved. However, the use of cultivation-based and -independent methods did provide complementary phylogenetic diversity overview of the Aquificales in these systems. Together, this survey revealed that the ELSC/VFR contains some of the largest diversity of Aquificales ever reported at a deep-sea vent area, that the diversity patterns are tied to the geography and geochemistry of the system, and that this geochemical diverse back-arc basin may harbor new members of the Aquificales. (C) 2014 Elsevier GmbH. All rights reserved.