Endogenous Steroids in the Rat Seminiferous Tubules. Comparison of the Stages of the Epithelial Cycle Isolated by Transillumination‐Assisted Microdissection
Endogenous Steroids in the Rat Seminiferous Tubules. Comparison of the Stages of the Epithelial Cycle Isolated by Transillumination‐Assisted Microdissection
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大鼠曲细精管中的内源性类固醇。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
A. Ruokonen
中科院分区：
文献类型：
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作者：
M. Parvinen;A. Ruokonen
描述了显微解剖程序，该程序允许在上皮周期的不同阶段分离大鼠生精小管的片段。该程序基于沿着新鲜分离的、未染色的生精小管的透照模式中与生精阶段相关的差异。它允许收集代表定义阶段的片段，其数量足以进行生化研究（5-10 毫克湿重）。
I、II-III、IV-V、VI、VIIa-b、VIIc-d、VIII、IX-XI、XII 和 XIII-XIV 阶段的管段已用于在 Lipidex-5000 层析后通过放射免疫测定法测量睾酮、5α-二氢睾酮、孕酮和 17α-羟基孕酮的内源浓度。睾酮是周期所有阶段最丰富的类固醇，其在第八阶段的浓度显着高于任何其他阶段。 5α-二氢睾酮的浓度明显较低，并且没有表现出类似的分布差异。孕酮和17α-羟基孕酮的水平在生精上皮周期的所有阶段都是相同的。
许多形态学和生化观察表明，生殖细胞和支持细胞在周期的第七和第八阶段的代谢活性发生了独特的变化。讨论了雄激素对这些变化的可能控制。
A microdissection procedure is described that allows the isolation of segments of rat seminiferous tubules in different stages of the epithelial cycle. This procedure is based on spermatogenic stage-dependent differences in the transillumination pattern along the freshly isolated, unstained seminiferous tubules. It allows collection of segments representing defined stages in amounts sufficient for biochemical studies (5–10 mg wet weight).
Tubular segments at stages I, II–III, IV–V, VI, VIIa–b, VIIc–d, VIII, IX–XI, XII, and XIII–XIV have been used for measurement of endogenous concentrations of testosterone, 5α-dihydrotestosterone, progesterone, and 17α-hydroxyprogesterone by radioimmunoassays after Lipidex-5000 chromatography. Testosterone was the most abundant steroid at all stages of the cycle, and its concentration at stage VIII was significantly higher than at any other stage. The concentration of 5α-dihydrotestosterone was markedly lower and did not exhibit similar differences in distribution. The levels of progesterone and 17α-hydroxyprogesterone were the same at all stages of the cycle of the seminiferous epithelium.
A number of morphologic and biochemical observations suggest unique changes in the metabolic activity of both germ cells and Sertoli cells at stages VII and VIII of the cycle. The possible androgen control of these changes is discussed.