Molecular characterization of disease-associated streptococci of the mitis group that are optochin susceptible
Molecular characterization of disease-associated streptococci of the mitis group that are optochin susceptible
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DOI：
10.1128/jcm.01137-06
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发表时间：
2006-11-01
影响因子：
9.4
通讯作者：
de la Campa, Adela G.
中科院分区：
文献类型：
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作者：
Balsalobre, Luz;Hernandez-Madrid, Antonia;de la Campa, Adela G.
在分子水平上对八种奥普托钦敏感 (Opt(s)) α-溶血 (viridans) 链球菌分离株进行了表征。这些分离株显示出草绿色链球菌和肺炎链球菌的典型表型特征。将这些分离株的管家基因序列与肺炎链球菌、轻症链球菌、口腔链球菌和假肺炎链球菌的管家基因序列进行比较表明，Opt(s) 分离株与轻症链球菌相对应。此外，Opts 链球菌经 Gen-Probe AccuProbe 肺炎球菌测试呈阴性，并与特定肺炎球菌探针（lytA 和 ply）以及 ant（大多数肺炎链球菌菌株中不存在的基因）杂交。此外，分离物不溶于1%脱氧胆酸钠，但完全溶解在0.1%脱氧胆酸钠中。 lytA 基因的序列分析表明，Opt(s) 链球菌携带 lytA 等位基因，这些等位基因存在于轻症群非肺炎球菌链球菌中。包含编码 F0F1 H+-ATPase 的 atp 操纵子的部分核苷酸序列的测定表明，分离株的奥普托钦敏感性是由于通过水平基因转移从肺炎链球菌获得了 atpC、atpA 和部分 atpB。
Eight optochin-susceptible (Opt(s)) allpha-hemolytic (viridans) streptococcus isolates were characterized at the molecular level. These isolates showed phenotypic characteristics typical of both viridans streptococci and Streptococcuspneumoniae. Comparison of the sequence of housekeeping genes from these isolates with those of S. pneumoniae, Streptococcus mitis, Streptococcus oralis, and Streptococcus pseudopneumoniae suggested that the Opt(s) isolates corresponded to streptococci of the mitis group. Besides, the Opts streptococci were negative by a Gen-Probe AccuProbe pneumococcus test and hybridized with specific pneumococcal probes (lytA and ply) but also with ant, a gene not present in most S. pneumoniae strains. Moreover, the isolates were insoluble in 1% sodium deoxycholate but completely dissolved in 0.1% deoxycholate. Sequence analysis of the lytA gene revealed that the Opt(s) streptococci carried lytA alleles characteristic of those present in nonpneumococcal streptococci of the mitis group. The determination of the partial nucleotide sequence embracing the atp operon encoding the F0F1 H+-ATPase indicated that the optochin susceptibility of the isolates was due to the acquisition of atpC, atpA, and part of atpB from S. pneumoniae by horizontal gene transfer.