Early life home microbiome and hyperactivity/inattention in schoolage children
Early life home microbiome and hyperactivity/inattention in schoolage children
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DOI：
10.1038/s41598-019-53527-1
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发表时间：
2019-11-22
影响因子：
4.6
通讯作者：
Heinrich, Joachim
中科院分区：
文献类型：
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作者：
Casas, Lidia;Karvonen, Anne M.;Heinrich, Joachim
本研究评估了生命早期的室内微生物多样性与 10 岁和 15 岁儿童的多动/注意力不集中症状之间的关联。从德国 LISA 出生队列中选择了富含 15 岁时多动/注意力不集中受试者的随机样本。在 3 个月大时收集卧室地板灰尘，并根据 Illumina MiSeq 测序数据计算 4 个细菌和真菌多样性指标 [观察到的操作分类单位 (OTU) 数量、Chao1、Shannon 和 Simpson 指数]。多动/注意力不集中基于 10 岁和 15 岁的优势和困难问卷（截止值 >= 7）。使用加权和调查逻辑回归模型研究了三分位数的 4 个多样性测量与多动/注意力不集中之间的调整关联。我们纳入了 226 名个体，他们掌握了微生物多样性和多动/注意力不集中的信息。生命早期细菌多样性与 10 岁时的多动/注意力不集中呈负相关[细菌 OTU（中 vs 低：aOR = 0.4，95%CI = (0.2-0.8））和 Chao1（中 vs 低：0.3 (0.1-0.5)；高 vs 低：0.3 (0.2-0.6)]，而真菌多样性直接相关 [Chao1（高 vs 低：aOR = 0.4，95%CI = (0.2-0.8)） 2.1 (1.1-4.0))、Shannon（中 vs 低：2.8 (1.3-5.8)）和 Simpson（中 vs 低：4.7 (2.4-9.3)）]。15 岁时，只有 Shannon 指数与多动/注意力不集中显着相关[细菌（中 vs 低：2.3 (1.2-4.2)；真菌（高 vs 低：0.5））。 (0.3-0.9))]。总之，生命早期接触微生物多样性可能在心理行为发展中发挥作用，我们观察到关联方向的异质性，鼓励进一步的纵向研究，以加深我们对观察到的关联背后的微生物群落特征的理解。
This study evaluates the association between indoor microbial diversity early in life and hyperactivity/inattention symptoms in children at ages 10 and 15 years.A random sample enriched with subjects with hyperactivity/inattention at age 15 years was selected from the German LISA birth cohort. Bedroom floor dust was collected at age 3 months and 4 bacterial and fungal diversity measures [number of observed operational taxonomic units (OTUs), Chao1, Shannon and Simpson indices] were calculated from Illumina MiSeq sequencing data. Hyperactivity/inattention was based on the Strengths and Difficulties Questionnaire at ages 10 and 15 (cut-off >= 7). Adjusted associations between 4 diversity measures in tertiles and hyperactivity/inattention were investigated with weighted and survey logistic regression models. We included 226 individuals with information on microbial diversity and hyperactivity/inattention. Early life bacterial diversity was inversely associated with hyperactivity/inattention at age 10 [bacterial OTUs (medium vs low: aOR = 0.4, 95%CI = (0.2-0.8)) and Chao1 (medium vs low: 0.3 (0.1-0.5); high vs low: 0.3 (0.2-0.6)], whereas fungal diversity was directly associated [Chao1 (high vs low: 2.1 (1.1-4.0)), Shannon (medium vs low: 2.8 (1.3-5.8)), and Simpson (medium vs low: 4.7 (2.4-9.3))]. At age 15, only Shannon index was significantly associated with hyperactivity/inattention [bacteria (medium vs low: 2.3 (1.2-4.2); fungi (high vs low: 0.5 (0.3-0.9))]. In conclusion, early life exposure to microbial diversity may play a role in the psychobehavioural development. We observe heterogeneity in the direction of the associations encouraging further longitudinal studies to deepen our understanding of the characteristics of the microbial community underlying the observed associations.