THE EFFECT OF FLUORIDE TREATMENT ON BONE-MINERAL CRYSTALS IN THE RAT
THE EFFECT OF FLUORIDE TREATMENT ON BONE-MINERAL CRYSTALS IN THE RAT
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DOI：
10.1016/8756-3282(92)90085-b
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发表时间：
1992-11-01
期刊：
影响因子：
4.1
通讯作者：
REY, C
中科院分区：
文献类型：
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作者：
GRYNPAS, MD;REY, C
为了研究氟对骨矿物晶体的影响，我们给雌性大鼠组8 mM NaF/L水和蒸馏水作为对照组。分别在6周、3个月和6个月处死大鼠。用中子活化法测定骨的氟含量。X射线衍射显示，氟处理的骨晶体尺寸/应变没有差异。傅立叶变换红外光谱（FTIR）显示，在氟化物喂养的动物，这似乎是与不稳定的磷酸盐环境的减少结晶度增加。在氟化和正常骨样品中发现了三条碳酸盐带。氟离子对碳酸根离子在A型和B型位点上的分布有很大影响。A型碳酸盐总是存在于骨中，但随着骨氟含量的增加而减少。在866 cm-1处发现的碳酸根谱带可能对应于氟化物与B型碳酸根离子的相互作用。最后，磷酸盐谱带被发现向高波数移动，这可能与氟离子引起的晶胞尺寸的变化有关。所有这些变化引起的氟降低骨晶体的溶解度，通过直接掺入磷灰石晶格中的氟离子，并通过减少不稳定的磷酸盐环境。
In order to investigate the effect of fluoride on bone mineral crystals, we gave groups of female rats 8 mM NaF/L water and distilled water to control groups. The rats were sacrificed at six weeks, three months, and six months. The fluor content of the bone was determined by neutron activation. X-ray diffraction showed no difference in bone crystal size/strain with fluoride treatment. Fourier transform Infrared Spectroscopy (FTIR) showed an increased crystallinity in fluoride-fed animals, which seems to be associated with a decrease of labile phosphate environment. Three carbonate bands have been found in fluoridated and normal bone samples. The distribution of carbonate ions on type A and B sites is strongly affected by fluoride. Type A carbonate is always present in bone, but decreases with increasing bone fluoride content. A carbonate band found at 866 cm-1 may correspond to a fluoride interaction with type B carbonate ions. Lastly, phosphate bands have been found to be shifted towards high wave number, which is probably related to the change in unit cell size induced by the fluoride ion. All these changes induced by fluoride reduce the solubility of bone crystals by direct incorporation of fluoride ions in the apatite lattice and by decreasing the labile phosphate environments.