Strain Differences in Susceptibility to 2‐Acetylaminofluorene and Phenobarbital Promotion of Rat Hepatocarcinogenesis in a Medium‐term Assay System: Quantitation of Glutathione S‐Transferase P‐positive Foci Development
Strain Differences in Susceptibility to 2‐Acetylaminofluorene and Phenobarbital Promotion of Rat Hepatocarcinogenesis in a Medium‐term Assay System: Quantitation of Glutathione S‐Transferase P‐positive Foci Development
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中期检测系统中对 2-乙酰氨基芴和苯巴比妥促进大鼠肝癌发生的敏感性的菌株差异：谷胱甘肽 S-转移酶 P 阳性病灶发育的定量
DOI：
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
S. Nagase
中科院分区：
文献类型：
--
作者：
M. Asamoto;H. Tsuda;M. Kagawa;J. D. de Camargo;N. Ito;S. Nagase
在我们实验室最初开发的中期生物测定系统中，使用雄性F344大鼠作为试验动物，胎盘型谷胱甘肽S-转移酶（GST-P）阳性病灶作为病变终点，检查了对肝脏致癌物2-乙酰氨基芴（2-AAF）和苯巴比妥（PB）促进敏感性的品系差异。在LEW、F344、NAR、SD、WBN、SHR、Wistar和ODS大鼠中比较了诱导的GST-P阳性肝细胞灶的数量和每平方厘米面积，这些大鼠以二乙基亚硝胺（DEN）开始给药，并在随后给予2-AAF或PB期间进行部分肝切除术。LEW、SD、WBN和F344大鼠对两种化合物的肝脏促进作用最敏感，在LEW病例中观察到2-AAF的病变面积增加了100倍。NAR和SHR株表现出中间的反应，而Wistar，特别是相关的ODS大鼠表现出非常低的耐受性。明显的菌株差异可以用2-AAF和PB以及DEN本身对测试的8个菌株中的每一个的促进作用的比较指数来表示。通过对DEN和2-AAF启动以及第二阶段促进刺激表现出的相对较高的敏感性，验证了F344大鼠用于生物测定模型。
Strain differences in susceptibility to promotion by the liver carcinogens 2‐acetylaminofluorene (2‐AAF) and phenobarbital (PB) were examined in the medium‐term bioassay system initially developed in our laboratory using male F344 rats as the test animal and glutathione S‐transferase placental form (GST‐P)‐positive foci as the lesion end‐point. Numbers and areas per cm2 of induced GST‐P‐positive hepatocellular foci were compared in LEW, F344, NAR, SD, WBN, SHR, Wistar and ODS rats initiated with diethylnitrosamine (DEN) and subjected to partial hepatectomy during subsequent administration of 2‐AAF or PB. LEW, SD, WBN, and F344 rats were most susceptible to hepatopromotion by both compounds, with a hundred fold increase in lesion area being observed for 2‐AAF in the LEW case. NAR and SHR strains demonstrated an intermediate response, while Wistar and, in particular, the related ODS rats demonstrated very low susceptibilities. The obvious strain differences could be expressed in terms of comparative indices of promoting effects of 2‐AAF and PB as well as DEN itself regarding each of the 8 strains tested. The use of F344 rats for the bioassay model was validated by the relatively high sensitivity to both DEN and 2‐AAF initiation as well as second‐stage promotion stimulus exhibited.
DOI：
10.1093/jnci/70.5.931
发表时间：
1983-05
期刊：
Journal of the National Cancer Institute
影响因子：
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作者：
A. Malkinson;D. S. Beer
通讯作者：
A. Malkinson;D. S. Beer
四种大鼠乳腺肿瘤易感性、乳腺细胞介导的诱变和多环芳烃代谢的关系。
DOI：
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发表时间：
1983
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
Moore,CJ;Bachhuber,AJ;Gould,MN
通讯作者：
Gould,MN
化学物质逐步发展癌症的细胞生物化学：G. H. A. Clowes 纪念讲座。
影响因子：
11.2
作者：
Farber,E
通讯作者：
Farber,E