BASIC FIBROBLAST GROWTH-FACTOR (BFGF) AND RAT C6 GLIOMA-CELLS - REGULATION OF EXPRESSION, ABSENCE OF RELEASE, AND RESPONSE TO EXOGENOUS BFGF
BASIC FIBROBLAST GROWTH-FACTOR (BFGF) AND RAT C6 GLIOMA-CELLS - REGULATION OF EXPRESSION, ABSENCE OF RELEASE, AND RESPONSE TO EXOGENOUS BFGF
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DOI：
10.1002/glia.440030610
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发表时间：
1990-01-01
期刊：
影响因子：
6.2
通讯作者：
UNSICKER, K
中科院分区：
文献类型：
--
作者：
WESTERMANN, R;UNSICKER, K
碱性成纤维细胞生长因子（bFGF）是一种有效的有丝分裂原的几种类型的细胞，包括神经胶质细胞，似乎也表达bFGF。我们以大鼠C6胶质瘤细胞为模型，研究了胶质瘤细胞bFGF的表达和释放，以及外源性bFGF对胶质瘤细胞的影响。我们已经表明，C6细胞，表达18 kD bFGF和几个更高分子量的免疫反应形式。bFGF的表达可被胎牛血清中存在的因子诱导。在其最初出现之后，bFGF以细胞密度依赖性方式调节。未发现C6细胞释放bFGF样免疫反应物质或bFGF样神经营养活性。外源性bFGF对C6细胞形态的改变与cAMP诱导的改变相似，但对C6细胞的增殖和生化分化无明显影响。从这些结果中，我们得出结论，C6细胞中的bFGF可能作为一种内源性（而不是自分泌）的有丝分裂原。bFGF在神经胶质细胞中的可能作用进行了讨论。
Basic fibroblast growth factor (bFGF) is a potent mitogen for several types of cells, including glial cells, which also seem to express bFGF. We have used rat C6 glioma cells as a model system to study the expression and release of bFGF by glioma cells, as well as the effects of exogenous bFGF on these cells. We have shown that C6 cells, express 18 kD bFGF and several higher molecular weight immunoreactive forms. The expression of bFGF could be induced by a factor present in fetal calf serum. Subsequent to its initial appearance, bFGF is regulated in a cell density‐dependent manner. Neither bFGF‐like immunoreactive material, nor bFGF‐like neurotrophic activity were found to be released by C6 cells. Exogenously applied bFGF changed C6 cell morphology similar to cyclic AMP induced alterations but had no significant influence on C6 cell proliferation and biochemical differentiation. From these results we conclude that bFGF in C6 cells might act as an endogenous (not autocrine) mitogen. Possible roles for bFGF in glial cells are discussed.