Sensitivity of Meloidogyne javanica and Tylenchulus semipenetrans to Isothiocyanates in Laboratory Assays.
Sensitivity of Meloidogyne javanica and Tylenchulus semipenetrans to Isothiocyanates in Laboratory Assays.
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DOI：
10.1094/phyto.2003.93.6.747
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发表时间：
2003-06
期刊：
影响因子：
3.2
通讯作者：
I. Zasada;H. Ferris
中科院分区：
文献类型：
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作者：
I. Zasada;H. Ferris
摘要异硫氰酸酯是十字花科植物产生的硫代葡萄糖苷的酶降解产物。硫代葡萄糖苷的分布因植物种类不同而不同，异硫氰酸酯衍生物对线虫的毒性也不同。从十字花科植物材料中释放出来的异硫氰酸酯对土壤中植物寄生线虫的控制一直不一致。了解各种异硫氰酸酯对线虫的相对毒性可能会提高成功率。通过实验室测定了7种市售异硫氰酸酯对半圆线虫和爪哇根结线虫的致死浓度(LC)值。2-苯乙基异硫氰酸酯和异硫氰酸苯酯的LC(90)值分别为0.01和0.03摩尔/毫升，异硫氰酸苯酯的LC(90)值分别为0.48和0.35摩尔/毫升。油菜来源的异硫氰酸苄酯或2-苯乙基异硫氰酸酯，以及次要程度的异硫氰酸烯丙酯，是植物寄生线虫治理的最有前途的候选者。这项研究的更广泛背景是开发一致和可靠地使用植物衍生化学物质进行线虫管理的方法。该策略是根据硫代葡萄糖苷图谱和目标线虫物种对相关异硫氰酸盐的敏感性来选择十字花科植物。
ABSTRACT Isothiocyanates are released through enzymatic degradation of glucosinolates produced by plants in the family Brassicaceae. Glucosinolate profiles differ among plant species and the isothiocyanate derivatives differ in their toxicity to nematodes. Control of plant-parasitic nematodes in soil by isothiocyanates released from incorporated brassicaceous plant material has been inconsistent. Success might be improved with knowledge of the relative toxicities of various isothiocyanates against nematodes. Laboratory assays were conducted to determine lethal concentration (LC) values in sand of seven commercially available isothiocyanates against Tylenchulus semipenetrans and Meloidogyne javanica. The LC(90) values were 0.01 and 0.03 mumol/ml for 2-phenylethyl isothiocyanate and 0.48 and 0.35 mumol/ml for phenyl isothiocyanate for T. semipenetrans and M. javanica, respectively. Brassicaceous sources of benzyl or 2-phenylethyl isothiocyanate and, to a lesser extent allyl isothiocyanate, are the most promising candidates for plant-parasitic nematode management. The broader context of this research is the development of approaches for consistent and reliable use of plant-derived chemicals for nematode management. The strategy is to select plants in the family Brassicaceae based on their glucosinolate profiles and the sensitivity of the target nematode species to the associated isothiocyanates.