Morphologic demonstration of two stages in the development of type II collagen-induced arthritis.
Morphologic demonstration of two stages in the development of type II collagen-induced arthritis.
复制标题
II 型胶原诱导的关节炎发展的两个阶段的形态学证明。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
D. Trentham
中科院分区：
文献类型：
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作者：
J. Caulfield;A. Hein;R. Dynesius;D. Trentham
对17只正常大鼠和38只II型胶原诱导性关节炎大鼠膝关节的胫骨、髌骨和股骨的髌下脂肪垫、纤维滑膜和软骨关节面进行了光镜和电镜观察。在正常动物中，覆盖脂肪垫的滑膜在结构上是可变的，即中皮层是扁平的，在中心区域有两到三层细胞厚，但在胫骨和髌骨末端是圆形的，有三到五层厚。脂肪垫两端的滑膜细胞中大空泡较多，且这些细胞中的内质网比中心区域的细胞少。纤维性滑膜由扁平的间皮组成，其细胞与脂肪垫中心区域的细胞相似。正常软骨未被间皮覆盖。用胶原免疫的动物的膝关节直到免疫后5天才显示出结构变化，此时可以通过免疫荧光在滑膜中检测到纤维蛋白。到第12天，在整个滑膜中发现纤维蛋白沉积，覆盖脂肪垫和纤维组织，以及软骨。此外，软组织上存在滑膜增生，滑膜细胞延伸到软骨上。第19天，在约20%的动物中，在覆盖软组织的滑膜内观察到由多形核白细胞和单核细胞混合物组成的炎性浸润。在软骨的边缘处也观察到内陷。免疫后30天检查80%没有细胞浸润的动物时，光学显微镜下既没有观察到增生，也没有观察到纤维蛋白沉积。到第45天，在20%的动物中，滑膜增生，在软组织中发现肉芽组织、瘢痕和肉芽肿。它的结论是，有两个不同的阶段，在这种滑膜疾病的发展-第一个特点是增生和纤维蛋白沉积，第二个炎性浸润的存在。
The infrapatellar fat pad, fibrous synovium, and cartilage joint surfaces of the tibia, patella, and femur of the knee joints of 17 normal rats and 38 rats with type II collagen-induced arthritis were examined by light and electron microscopy. In the normal animals, the synovium covering the fat pad was structurally variable in that the mesothelium was flattened and two to three cell layers thick in the central region but rounded and three to five layers thick at the tibial and patellar ends. Large vacuoles were more numerous in the synovial cells at either end of the fat pad, and these cells contained less endoplasmic reticulum than those in the central region. The fibrous synovium consisted of a flattened mesothelium composed of cells similar to those found in the central region of the fat pad. The normal cartilage was not covered by a mesothelium. Knees from animals immunized with collagen showed no structural changes until 5 days after immunization, when fibrin could be detected in the synovium by immunofluorescence. By day 12, fibrin deposition was found throughout the synovium covering the fat pad and fibrous tissue, as well as over the cartilage. In addition, hyperplasia of the synovium over the soft tissues was present, and synovial cells extended over the cartilage. On day 19, in approximately 20 per cent of the animals, inflammatory infiltrates, composed of mixtures of polymorphonuclear leukocytes and mononuclear cells, were observed within the synovium covering the soft tissues. Infiltrates were also seen at the edges of the cartilage. When the 80 per cent of the animals that did not have cellular infiltrates were examined 30 days after immunization, neither hyperplasia nor fibrin deposition were seen by light microscopy. By day 45, in 20 per cent of the animals, the synovium was hyperplastic, and granulation tissue, scarring, and granulomata were found in the soft tissues. It is concluded that there are two distinct stages in the development of this synovial disease--the first is characterized by hyperplasia and fibrin deposition, and the second by the presence of inflammatory infiltrates.