Improve uridine production by modifying related metabolic pathways in Bacillus subtilis
Improve uridine production by modifying related metabolic pathways in Bacillus subtilis
复制标题
DOI：
10.1007/s10529-020-02820-5
复制
发表时间：
2020-01
影响因子：
2.7
通讯作者：
Xueting Zhang;Chen Wang;Lu Liu;R. Ban
中科院分区：
文献类型：
--
作者：
Xueting Zhang;Chen Wang;Lu Liu;R. Ban
目的对枯草芽孢杆菌中与尿苷产生有关的代谢途径进行改造，以提高尿苷的产量。结果将枯草芽孢杆菌TD300中葛根素的相对转录水平降低到80%，并使衍生菌株TD312的产量提高到11.81g尿苷/L，产量提高到270g尿苷/g葡萄糖。PccpA原位表达pucR基因，使pucR基因相对转录水平增加194.01倍，操纵子相对转录水平增加349.71倍。结论利用基因工程枯草杆菌摇瓶发酵生产尿苷/L超过13 mg/g，尿苷/g葡萄糖产量达250 mg/g葡萄糖。
ObjectivesThe metabolic pathway related to uridine production was modified inBacillus subtilisin order to increase the production of uridine.ResultsDecreasing the relative transcriptional level ofpuroperon inBacillus subtilisTD300 to 80%, and the production of the derived strain TD312 was increased to 11.81 g uridine/l and the yield was increased to 270 mg uridine/g glucose. The expression ofpucRgene in situ by PccpAresulting in a 194.01-fold increase in the relative transcriptional level ofpucRgene and 349.71-fold increase in the relative transcriptional level ofureoperon, respectively. Furthermore, the production of TD314 reached 13.06 g uridine/l, while the yield reached 250 mg uridine/g glucose.ConclusionThis is the first report that more than 13 g uridine/l with a yield of 250 mg uridine/g glucose is produced in shake flask fermentation of genetically engineeredBacillus subtilis.