Prediction of precision from signal and noise measurement in liquid chromatography: Limit of detection
Prediction of precision from signal and noise measurement in liquid chromatography: Limit of detection
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液相色谱中信号和噪声测量的精度预测：检测限
DOI：
10.1007/bf02688002
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
R. B. Poe
中科院分区：
文献类型：
--
作者：
Y. Hayashi;R. Matsuda;R. B. Poe
介绍了一种高效液相色谱法检测限（LOD）的方法。以白噪声和马尔可夫过程混合随机过程为模型，采用单纯形最小二乘法拟合仪器基线变化的功率谱密度。白噪声的特征为标准差（SD）， ~ w；通过SD （~ m）和自相关度ρ (ρ)来描述马尔可夫过程。计算LOD信号所需的所有参数均通过实验获得，无需重复测量。不需要任意常数。LOD信号是唯一确定的，其特征是分析物测量的相对标准偏差（RSD）为33.3%，误差为第一种方法的0.13%。该信号还规定了信噪比=3，使用定义噪声源于白噪声和马尔可夫过程。利用实际基线和峰值进行蒙特卡罗模拟，验证了理论结论。给出了萘、苊、芘和苝的LOD浓度。
A method to determine the limit of detection (LOD) in high performance liquid chromatography (HPLC) is described. The power spectral density of instrumental baseline variation is fitted by the simplex least squares methods with a mixed random process of white noise and Markov process as a model. The white noise is characterized by standard deviation (SD), ∼w; the Markov process by the SD, ∼m, and auto-correlation degree, ρ. All required parameters for calculating the LOD signal are obtained by experiment without repeat measurements. No arbitrary constants are needed. The LOD signal is uniquely determined and is characterized by 33.3% relative standard deviation (RSD) of analyte measurements and 0.13% of the error of the first type. This signal also specifies that the signal-to-noise ratio=3, using the definition of noise originating from the white noise and Markov process. The theoretical conclusion is verified by the Monte Carlo simulation using real baseline and peaks. The LOD concentrations for naphthalene, acenaphthene, pyrene and perylene are given.