Mice with the deleted neurofilament of low molecular weight (Nefl) gene:: 2.: Effects on motor functions and spatial orientation
Mice with the deleted neurofilament of low molecular weight (Nefl) gene:: 2.: Effects on motor functions and spatial orientation
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DOI：
10.1002/jnr.20493
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发表时间：
2005-06-15
影响因子：
4.2
通讯作者：
Lalonde, R
中科院分区：
文献类型：
--
作者：
Dubois, M;Strazielle, C;Lalonde, R
NO基因零突变的小鼠在运动活动、平衡和空间定向测试中与正常对照组进行比较。尽管有正常的行走能力，但NFL-/-小鼠在开阔的场地上后背更少，在静止的横梁上跨越的节段更少，当悬挂在单杠上时，摔倒的频率更高。此外，在训练开始时，NFL-/-小鼠在Morris水迷宫中获得位置学习时，在到达逃生平台之前的游泳距离比对照组要大。运动障碍与小脑和脑干细胞色素氧化酶活性升高呈线性相关。这些结果表明，早在6个月时，NFL蛋白的耗尽就足以导致轻微的感觉运动功能障碍和空间缺陷，但没有明显的瘫痪迹象。(C)2005年Wiley-Liss，Inc.
Mice with a null mutation of the NO gene were compared with normal controls in tests of motor activity, equilibrium, and spatial orientation. Despite a normal capacity to ambulate, NFL -/- mice had fewer rears in an open field, crossed fewer segments on stationary beams, and fell more frequently when suspended on a horizontal bar. In addition, the distance swum before reaching the escape platform was greater in NFL -/- mice than in controls during acquisition of place learning in the Morris water maze at the start of training. The motor impairments were linearly correlated with increased cytochrome oxidase activity seen in cerebellum and brainstem. These results indicate that, as early as 6 months, depletion of the NFL protein is sufficient to cause mild sensorimotor dysfunctions and spatial deficits, but without overt signs of paresis. (c) 2005 Wiley-Liss, Inc.