Ultrastructural observations on mitochondria and microvesicles in renal oncocytoma, chromophobe renal cell carcinoma, and eosinophilic variant of conventional (clear cell) renal cell carcinoma
Ultrastructural observations on mitochondria and microvesicles in renal oncocytoma, chromophobe renal cell carcinoma, and eosinophilic variant of conventional (clear cell) renal cell carcinoma
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DOI：
10.1097/00000478-200009000-00008
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发表时间：
2000-09-01
影响因子：
5.6
通讯作者：
Amin, MB
中科院分区：
文献类型：
--
作者：
Tickoo, SK;Lee, MW;Amin, MB
在光镜下，肾嗜酸细胞瘤、嗜铬肾细胞癌和常规(透明细胞)肾细胞癌中颗粒状嗜酸细胞质的形态重叠特征可能给诊断带来困难。我们观察了5例肾嗜酸细胞瘤、7例嗜铬肾细胞癌和5例常规(透明细胞)肾细胞癌的超微结构。特别注意线粒体和微泡及其相互关系。电子显微镜特征与光镜下表现相一致。所有的谣言都有丰富的线粒体。虽然所有的嫌色肾细胞癌中都有大量的微泡，但在肾嗜酸细胞瘤(2/5)和常规(透明细胞)肾细胞癌的嗜酸性变异型(1/5)中，微泡的数量有时也很少。所研究的三种肾肿瘤的线粒体在形态上都不同，在肾嗜酸细胞瘤中线粒体以均匀和圆形为主，在嗜铬肾细胞癌中以板层脊为主，在嫌色肾细胞癌中线粒体形状和大小可变，以管囊性脊为主，在常规(透明细胞)肾细胞癌中，线粒体肿胀和多形性，基质稀疏，脊变细。在所有的嫌色肾细胞癌中，数量可变的线粒体都有外膜突起，其中一些外膜内有部分内膜。这些突起与附近的细胞质微泡非常相似，线粒体的管囊状突起也是如此。有些微泡含有均匀的、电子致密的、颗粒细小的基质，类似于线粒体中的基质。在7例嫌色肾细胞癌中，1例在粗面内质网内可见微泡，另2例在某些囊泡内可见线粒体。这些特征强烈地表明了微泡和线粒体之间的密切关系。基于囊泡形成在正常线粒体发生中的作用，以及我们的一些观察结果，我们认为线粒体发生缺陷可能是嫌色肾癌微泡的来源。
On light microscopic examination, the morphologically overlapping features of granular eosinophilic cytoplasm in renal oncocytoma and the eosinophilic variants of chromophobe renal cell carcinoma and conventional (clear cell) renal cell carcinoma may pose difficulties in diagnosis. We investigated the ultrastructure of 5 renal oncocytomas, 7 eosinophilic variants of chromophobe renal cell carcinoma, and 5 eosinophilic variants of conventional (clear cell) renal cell carcinoma. Special attention was paid to mitochondria and microvesicles and interrelations thereof. The electron microscopic Features were correlated with the light microscopic findings. All of the rumors had abundant mitochondria. Although abundant microvesicles were present in all of the chromophobe renal cell carcinomas, scant numbers of microvesicles were also sometimes present in renal oncocytomas (2 of 5) and in the eosinophilic variant of conventional (clear cell) renal cell carcinoma (1 of 5). The mitochondria in all three types of renal neoplasms studied differed in morphology, being predominantly uniform and round with predominantly lamellar cristae in renal oncocytoma, variable in shape and size with predominantly tubulocystic cristae in chromophobe renal cell carcinoma, and swollen and pleomorphic with rarefied matrix and attenuated cristae in the eosinophilic variant of conventional (clear cell) renal cell carcinoma. Variable numbers of mitochondria in all of the chromophobe renal cell carcinomas had outpouchings of the outer membranes, some of which carried parts of inner membrane within them. These outpouchings closely resembled the nearby cytoplasmic microvesicles, as did the tubulocystic cristae of the mitochondria. Some microvesicles contained homogeneous, electron-dense, finely granular matrix, similar to that seen in mitochondria. In one of seven chromophobe renal cell carcinomas, microvesicles were present in rough endoplasmic reticulum, and in two others, mitochondria were present within some vesicles. These features strongly suggest a close relationship between the microvesicles and mitochondria. Based on the role of vesicle formation in normal mitochondriogenesis, and some of our observations, we propose that defective mitochondriogenesis may be the source of microvesicles in chromophobe renal cell carcinomas.