Seed-specific transcription factors ABI3 and FUS3:: molecular interaction with DNA
Seed-specific transcription factors ABI3 and FUS3:: molecular interaction with DNA
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DOI：
10.1007/s00425-004-1206-9
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发表时间：
2004-05-01
期刊：
影响因子：
4.3
通讯作者：
Conrad, U
中科院分区：
文献类型：
--
作者：
Mönke, G;Altschmied, L;Conrad, U
拟南芥（L.）Heynh。种子特异性转录因子ABI3和FUS3在成熟种子发育过程中起关键调控作用。高度保守的RY基序[DNA基序CATGCA(TG)]存在于许多种子特异性启动子中，是这两种调控因子的重要靶点。在这里，我们表明，在体外，全长ABI3蛋白和FUS3蛋白能够与RY- dna结合，并且这两种转录因子的B3结构域对于与RY元件的特异性相互作用是必要和充分的。RY基序的侧翼序列调节这种结合，但RY序列的单独存在允许ABI3和FUS3在体外与靶标特异性相互作用。ABI3和FUS3的转录活性，通过瞬时启动子激活来测量，需要B3 dna结合域和一个激活域。除了已知的位于n末端的激活域外，在ABI3的B1区发现了第二个转录激活域。
In Arabidopsis thaliana (L.) Heynh. the seed-specific transcription factors ABI3 and FUS3 have key regulatory functions during the development of mature seeds. The highly conserved RY motif [DNA motif CATGCA(TG)], present in many seed-specific promoters, is an essential target of both regulators. Here we show that, in vitro, the full-length ABI3 protein, as well as FUS3 protein, is able to bind to RY-DNA and that the B3 domains of both transcription factors are necessary and sufficient for the specific interaction with the RY element. Flanking sequences of the RY motif modulate the binding, but the presence of an RY sequence alone allows the specific interaction of ABI3 and FUS3 with the target in vitro. Transcriptional activity of ABI3 and FUS3, measured by transient promoter activation, requires the B3 DNA-binding domain and an activation domain. In addition to the known N-terminal-located activation domain, a second transcription activation domain was found in the B1 region of ABI3.