The response of endothelial cells to fluid shear stress using a co-culture model of the arterial wall
The response of endothelial cells to fluid shear stress using a co-culture model of the arterial wall
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DOI：
10.1080/10623320210714
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发表时间：
2002-01-01
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影响因子：
--
通讯作者：
Remuzzi, A
中科院分区：
文献类型：
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作者：
Imberti, B;Seliktar, D;Remuzzi, A
采用动脉壁内皮细胞（EC）和平滑肌细胞（SMC）共培养模型，研究流体切应力对EC行为的影响。这个模型，除了是一个更现实的组织模拟，是一个有价值的研究工具，研究机械刺激后的SMC和EC的行为。在本研究中，使用10%的循环应变来改变SMC接种的胶原凝胶的特性。这种形式的应变预处理导致血管壁的重排，产生周向取向的细胞和胶原纤维。随后，将预处理的胶原凝胶与EC接种，并暴露于流体诱导的剪切应力（10达因/cm（2））48小时。在没有流动的情况下，接种在平板结构上的EC与下面的胶原纤维一起定向。剪切结构显示EC沿血流方向定向。剪切应力也影响EC增殖，与未剪切的结构相比，分裂EC的总数减少了48%。剪切诱导的增殖减少进一步增强时，构建体第一应变预处理（64%的减少）。此外，切应力实验的条件培养基抑制接种在组织培养塑料上的EC的增殖。这些结果表明，EC对胶原共培养模型中的流体剪切应力的响应受到底层基质的影响，并且在本研究中，通过应变预处理来修改。
An endothelial cell (EC) smooth muscle cell (SMC) co-culture model of the arterial wall was used to study the effect of fluid shear stress on EC behavior. This model, in addition to being a more realistic tissue analogue, is a valuable research tool for studying the effects of mechanical stimulation upon the behavior of both SMCs and ECs. In the present study, a 10% cyclic strain was used to alter the characteristics of an SMC-seeded collagen gel. This form of strain preconditioning resulted in a rearrangement of the vessel wall that yielded circumferentially oriented cells and collagen fibrils. The preconditioned collagen gel was subsequently seeded with ECs and exposed to fluid-induced shear stress (10 dynes/cm(2)) for 48 hr. In the absence of flow, ECs seeded on slab constructs were oriented with the underlying collagen fibrils. Sheared constructs exhibited ECs oriented in the flow direction. Shear stress also affected EC proliferation, reducing the total number of dividing ECs by as much as 48 percent compared to unsheared constructs. The shear-induced reduction in proliferation was further enhanced when constructs were first strain-preconditioned (64% reduction). Moreover, conditioned media from shear stress experiments inhibited proliferation of ECs seeded on tissue culture plastic. These results suggest that EC response to fluid shear stress in a collagen co-culture model is influenced by the underlying substrate, and one that in this study is modified by strain preconditioning.