MiMIF-2 Effector of Meloidogyne incognita Exhibited Enzyme Activities and Potential Roles in Plant Salicylic Acid Synthesis
MiMIF-2 Effector of Meloidogyne incognita Exhibited Enzyme Activities and Potential Roles in Plant Salicylic Acid Synthesis
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DOI：
10.3390/ijms21103507
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发表时间：
2020-05
影响因子：
5.6
通讯作者：
Jianlong Zhao;Z. Mao;Qinghua Sun;Qian Liu;Heng Jian;B. Xie
中科院分区：
文献类型：
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作者：
Jianlong Zhao;Z. Mao;Qinghua Sun;Qian Liu;Heng Jian;B. Xie
植物寄生线虫通过分泌一系列效应物调节宿主免疫力来促进其寄生，但具体的分子机制尚不清楚。动物寄生虫分泌巨噬细胞移动抑制因子（MIF）样蛋白以逃避宿主免疫系统，其生化活性在其中起重要作用。已有研究表明，MiMIF-2效应子由南方根结线虫分泌，通过与膜联蛋白相互作用调节宿主免疫。在这项研究中，我们表明MiMIF-2具有互变异构酶活性，并保护线虫免受H2 O2损伤。MiMIF-2的表达不仅降低了线虫感染拟南芥过程中H2 O2的产生量，而且通过抑制活性氧的爆发抑制了线虫诱导的本氏烟草细胞死亡。RNA-seq转录组分析和RT-qPCR结果表明，在转MiMIF-2基因的拟南芥中，部分热激蛋白的表达量下调。经flg 22处理后，RNA-seq转录组分析表明，miMIF-2基因在拟南芥中的差异表达基因主要集中在植物激素信号转导、化合物代谢和植物防御等方面。RT-qPCR和代谢组学结果证实，水杨酸（SA）相关标记基因和SA含量显著降低。我们的研究结果为MiMIF-2如何调节植物免疫力提供了全面的理解，并拓宽了对M. incognita和寄主植物。
Plant-parasitic nematodes secrete a series of effectors to promote parasitism by modulating host immunity, but the detailed molecular mechanism is ambiguous. Animal parasites secrete macrophage migration inhibitory factor (MIF)-like proteins for evasion of host immune systems, in which their biochemical activities play essential roles. Previous research demonstrated that MiMIF-2 effector was secreted by Meloidogyne incognita and modulated host immunity by interacting with annexins. In this study, we show that MiMIF-2 had tautomerase activity and protected nematodes against H2O2 damage. MiMIF-2 expression not only decreased the amount of H2O2 generation during nematode infection in Arabidopsis, but also suppressed Bax-induced cell death by inhibiting reactive oxygen species burst in Nicotiana benthamiana. Further, RNA-seq transcriptome analysis and RT-qPCR showed that the expression of some heat-shock proteins was down regulated in MiMIF-2 transgenic Arabidopsis. After treatment with flg22, RNA-seq transcriptome analysis indicated that the differentially expressed genes in MiMIF-2 expressing Arabidopsis were pointed to plant hormone signal transduction, compound metabolism and plant defense. RT-qPCR and metabolomic results confirmed that salicylic acid (SA) related marker genes and SA content were significantly decreased. Our results provide a comprehensive understanding of how MiMIF-2 modulates plant immunity and broaden knowledge of the intricate relationship between M. incognita and host plants.