The general secrotory pathway of Burkholderia gladioli pv. agaricicola BG164R is necessary for for cavity disease in white button mushrooms
The general secrotory pathway of Burkholderia gladioli pv. agaricicola BG164R is necessary for for cavity disease in white button mushrooms
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DOI：
10.1128/aem.72.5.3558-3565.2006
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发表时间：
2006-05-01
影响因子：
4.4
通讯作者：
Heinemann, Jack A.
中科院分区：
文献类型：
--
作者：
Chowdhury, Piklu Roy;Heinemann, Jack A.
白色蘑菇的空洞病是由唐菖蒲伯克霍尔德氏菌pv.伞菌。我们描述了菌株BG164R的6个突变体的分离和表征，这些突变体不再引起蘑菇的这种疾病。这些突变被定位到一般分泌途径（GSP）的基因。这是第一次报道11型分泌途径与蘑菇疾病的关联。六个无毒突变体的表型如下：不能降解蘑菇组织，分泌几丁质酶和蛋白酶的能力大大降低，鞭毛数量减少。使用这些突变体，我们还进行了新的观察，即引起蘑菇组织降解的因素，从而导致腔病的表达，可以从菌丝体抑制中分离出来，因为无毒突变体继续抑制活跃生长的蘑菇菌丝体的生长。B的GSP位点。唐菖蒲随后被克隆和定位，并与密切相关物种的相同基因座进行比较，确定了B的gsp操纵子的遗传结构。剑兰湾与该属其它种的一致。我们还确定了最常见的土著细菌种群存在于蘑菇子实体从新西兰农场，其中之一，Ewingella americana，被发现是一个明显的拮抗剂B。剑兰湾伞菌。虽然其他研究人员已经报告了由于其他蘑菇疾病中内源性和致病细菌之间的相互作用而增强的疾病症状，但据我们所知，这是第一次报告拮抗作用。
Cavity disease in white button mushrooms is caused by Burkholderia gladioli pv. agaricicola. We describe the isolation and characterization of six mutants of the strain BG164R that no longer cause this disease on mushrooms. The mutations were mapped to genes of the general secretory pathway (GSP). This is the first report of the association of the type 11 secretion pathway with a disease in mushrooms. Phenotypes of the six avirulent mutants were the following: an inability to degrade mushroom tissue, a highly reduced capacity to secrete chitinase and protease, and a reduced number of flagella. Using these mutants, we also made the novel observation that the factors causing mushroom tissue degradation, thereby leading to the expression of cavity disease, can be separated from mycelium inhibition because avirulent mutants continued to inhibit the growth of actively growing mushroom mycelia. The GSP locus of B. gladioli was subsequently cloned and mapped and compared to the same locus in closely related species, establishing that the genetic organization of the gsp operon of B. gladioli pv. agaricicola is consistent with that of other species of the genus. We also identify the most common indigenous bacterial population present in the mushroom fruit bodies from a New Zealand farm, one of which, Ewingella americana, was found to be an apparent antagonist of B. gladioli pv. agaricicola. While other investigators have reported enhanced disease symptoms due to interactions between endogenous and disease-causing bacteria in other mushroom diseases, to the best of our knowledge this is the first report of an antagonistic effect.