Estrogen receptors α and β in the normal, hyperplastic and carcinomatous human prostate
Estrogen receptors α and β in the normal, hyperplastic and carcinomatous human prostate
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DOI：
10.1677/joe.0.1680447
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发表时间：
2001-03-01
影响因子：
4
通讯作者：
Paniagua, R
中科院分区：
文献类型：
--
作者：
Royuela, M;de Miguel, MP;Paniagua, R
已经描述了两种不同的雌激素受体（ER-α和ER-β），其差异性地参与调节生殖组织的正常功能。ER-α长期以来被认为是唯一的雌激素受体，并且它已在人前列腺的基质细胞中检测到，但在上皮中未检测到。为了获得两种受体类型的差异效应的新信息，我们已经研究了它们在正常前列腺，良性前列腺增生（BPH），前列腺癌（PC）的免疫组化，ELISA和Western印迹的本地化。上皮细胞免疫染色在正常前列腺中是不存在的，存在于BPH（10%的细胞）和PC（80%的细胞），而约15%的基质细胞在研究的三种类型的前列腺标本中ER-α呈阳性免疫染色。在正常前列腺（13%的细胞）、BPH（30%的细胞）和PC（79%的细胞）中检测到ER-β的上皮免疫染色，而在正常和增生性前列腺中不存在ER-β的基质免疫染色，并且存在于PC（12%的细胞）中。正常前列腺中两种受体类型（上皮中的ER-β和间质中的ER-α）的互补存在可能解释了BPH发展中雌激素作用的机制。在BPH和PC中，ER-α和ER-β的上皮免疫染色增加，表明雌激素受体参与增生和癌症主要涉及上皮。
Two different estrogen receptors (ER-alpha and ER-beta) have been described, which are differentially involved in regulating the normal function of reproductive tissues. ER-alpha was considered for a long time to be the only estrogen receptor, and it has been detected in the stromal cells of the human prostate but not in the epithelium. To obtain new information about the differential effects of both receptor types, we have investigated their localization in normal prostates, benign prostatic hyperplasia (BPH), and prostatic cancer (PC) by immunohistochemistry, ELISA and Western blot. Epithelial immunostaining was absent in normal prostates and was present in BPH (10% of cells) and PC (80% of cells), whereas about 15% of stromal cells were positively immunostained for ER-alpha in the three types of prostatic specimens studied. Epithelial immunostaining for ER-beta was detected in normal prostates (13% of cells), BPH (30% of cells) and PC (79% of cells), whereas stromal immunostaining for ER-beta was absent in normal and hyperplastic prostates and was present in PC (12% of cells). The complementary presence of both receptor types in the normal prostate (ER-beta in the epithelium and ER-alpha in the stroma) might explain the mechanism of estrogen action in the development of BPH. The increased epithelial immunostaining for both ER-alpha and ER-beta in BPH and PC suggests that the involvement of estrogen receptors in hyperplasia and cancer concerns mainly the epithelium.