A quantitative theory model of a photobleaching mechanism
A quantitative theory model of a photobleaching mechanism
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DOI：
10.1088/0256-307x/20/11/010
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发表时间：
2003-11-01
影响因子：
3.5
通讯作者：
Luo, QM
中科院分区：
文献类型：
--
作者：
Chen, TS;Zeng, SQ;Luo, QM
提出了一个光漂白模型：D-P（染料-光子相互作用）和D-O（染料-氧氧化反应）光漂白理论。获得了单光子和双光子激发（I PE和TPE）下光漂白速率的定量功率依赖性。该模型可以很好地解释我们和其他实验结果。在不饱和条件下，TPE对罗丹明B的光漂白速率的实验研究表明，光漂白速率的幂次依赖性随染料浓度的增加而增加，单个分子的光漂白速率以激发光强度的二次方增加，这与系综分子的高阶（> 3）非线性依赖性不同。
A photobleaching model: D-P (dye-photon interaction) and D-O (Dye-oxygen oxidative reaction) photobleaching theory, is proposed. The quantitative power dependences of photobleaching rates with both one- and two-photon excitations (I PE and TPE) are obtained. This photobleaching model can be used to elucidate our and other experimental results commendably. Experimental studies of the photobleaching rates for rhodamine B with TPE under unsaturation conditions reveals that the power dependences of photobleaching rates increase with the increasing dye concentration, and that the photobleaching rate of a single molecule increases in the second power of the excitation intensity, which is different from the high-order (> 3) nonlinear dependence of ensemble molecules.