The Edar subfamily in feather placode formation
The Edar subfamily in feather placode formation
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DOI：
10.1016/j.ydbio.2007.02.011
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发表时间：
2007-05-01
影响因子：
2.7
通讯作者：
Headon, Denis J.
中科院分区：
文献类型：
--
作者：
Drew, Caroline F.;Lin, Chili Min;Headon, Denis J.
TNF受体家族的一个亚群，由Edar、Troy和Xedar组成，与外胚层附属物（如毛囊、牙齿和汗腺）的发育有关。我们已经分离出这三种受体的鸡同源物，并分析了它们在早期羽毛发育中的作用。哺乳动物和鸟类之间的蛋白质序列的保守性是可变的，鸟类的Edar显示出最大程度的序列一致性。雪松与其哺乳动物同源物的不同之处在于它包含一个细胞内死亡结构域。所有这三种受体在羽毛早期形态发生时都有表达，并且每种受体的显性阴性形式都会损害上皮对羽毛芽形态发生的贡献，而真皮对羽毛芽形态发生的贡献似乎不受影响。每个受体的过度激活导致更广泛的基板命运假设，尽管在皮肤的不同区域。受体信号集中于NF-kappa B，抑制该转录因子会以特定阶段的方式改变羽毛芽的数量和大小。我们的研究结果说明了这三种受体在鸟类皮肤形态发生中的作用，也表明羽毛基板命运的激活因子通过相互调节来决定皮肤附属物的位置和大小。(c) 2007爱思唯尔公司版权所有。
A subgroup of the TNF receptor family, composed of Edar, Troy and Xedar, are implicated in the development of ectodermal appendages, such as hair follicles, teeth and sweat glands. We have isolated chicken orthologues of these three receptors and analysed their roles in early feather development. Conservation of protein sequences between mammalian and avian proteins is variable, with avian Edar showing the greatest degree of sequence identity. cXedar differs from its mammalian orthologue in that it contains an intracellular death domain. All three receptors are expressed during early feather morphogenesis and dominant negative forms of each receptor impair the epithelial contribution to feather bud morphogenesis, while the dermal contribution appears unaffected. Hyperactivation of each receptor leads to more widespread assumption of placode fate, though in different regions of the skin. Receptor signaling converges on NF-kappa B, and inhibiting this transcription factor alters feather bud number and size in a stage-specific manner. Our findings illustrate the roles of these three receptors during avian skin morphogenesis and also suggest that activators of feather placode fate undergo mutual regulation to reach a decision on skin appendage location and size. (c) 2007 Elsevier Inc. All rights reserved.