UPLC-MS Analysis for Simultaneous Determination of Eight Components in the 'Ge-Gen-Qin-Lian' Tablets
UPLC-MS Analysis for Simultaneous Determination of Eight Components in the 'Ge-Gen-Qin-Lian' Tablets
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DOI：
10.1365/s10337-009-1002-6
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发表时间：
2009-05-01
期刊：
影响因子：
1.7
通讯作者：
Wang, Xinhong
中科院分区：
文献类型：
--
作者：
An, Rui;Wang, Ying;Wang, Xinhong
A novel and rapid ultra performance liquid chromatography-mass spectrometry method was developed and validated for simultaneous determination of eight constituents in 'Ge-Gen-Qin-Lian' tablets. A UPLC BEH C(18) column (2.1 x 100 mm, 1.7 mu m) was utilized in this method, and a mixture of methanol, 0.1% formic acid, and 5 mmol L(-1) ammonium acetate was adopted in the gradient elution program. The mass spectrometer equipped with an electrospray ionization source was performed in the selected-ion reaction (SIR) mode. A good linearity correlation (R (2) > 0.9991) was obtained over the investigated concentration ranges. Recoveries of this method for the eight components were from 96.00 to 100.6% (RSD % < 3.4%).
