MAPPING COMPONENTS OF PARTIAL RESISTANCE TO NORTHERN LEAF-BLIGHT OF MAIZE USING RECIPROCAL TRANSLOCATIONS
MAPPING COMPONENTS OF PARTIAL RESISTANCE TO NORTHERN LEAF-BLIGHT OF MAIZE USING RECIPROCAL TRANSLOCATIONS
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DOI：
10.1094/phyto-82-225
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发表时间：
1992-02-01
期刊：
影响因子：
3.2
通讯作者：
WICKS, ZW
中科院分区：
文献类型：
--
作者：
BREWSTER, VA;CARSON, ML;WICKS, ZW
以玉米大斑病菌（Exserohilum turcicum）引起的北方大斑病（NLB）为感病材料，以部分抗病自交系Mo17为母本，经自交1代，构建玉米F2群体。 用糯性（wx）隐性基因标记易位断裂点，并在种植前将F2种子分为糯性或非糯性。 F2代植株接种E.在田间和温室中评价了玉米大斑病菌的侵染效率、潜伏期、病斑长度、病斑扩展速率和孢子形成。 还评估了田间实验中F2植物在成年植物上的疾病严重性。 使用Student t检验比较蜡质和非蜡质F2植物的评级，以确定参与相互易位的染色体臂中的一个或两个是否携带用于NLB抗性的基因。 Mo17的部分抗性（以田间成年植株的疾病严重程度表示）始终与3号染色体、4号染色体短臂和6号染色体长臂上的因子相关。 温室生长的幼苗的部分电阻的组件的措施很少是显着的，并不符合成年植物在该领域的疾病严重程度的评级。 基于对田间较老但仍营养生长的植物的测量，4号染色体短臂和6号染色体长臂上的一个或多个基因似乎与病变数量的减少和潜伏期的增加有关，这两者都与疾病的严重程度相关。 病变长度，病变扩张率和孢子形成强度与Mo17的部分抗性无关。 部分抗性组分在幼苗中表达的不一致性可能是由于抗性基因的环境和发育调控。
Maize F2 populations were constructed by crossing a series of northern leaf blight (NLB, caused by Exserohilum turcicum) susceptible reciprocal translocation stocks with the partially resistant inbred line Mo17, followed by one generation of selfing. Translocation breakpoints were marked with the waxy (wx) recessive gene and F2 seed were classified as waxy or nonwaxy before planting. F2 plants inoculated with E. turcicum were evaluated in the field and greenhouse for infection efficiency, incubation period, lesion length, lesion expansion rate, and sporulation. F2 plants in field experiments were also evaluated for disease severity on adult plants. Ratings of waxy and nonwaxy F2 plants were compared using Student's t tests to determine if one or both of the chromosome arms involved in the reciprocal translocation carried a gene or genes for NLB resistance. Partial resistance (as expressed by disease severity on adult plants in the field) in Mo17 was consistently associated with factors on chromosome 3, the short arm of chromosome 4, and the long arm of chromosome 6. Measures of components of partial resistance on greenhouse-grown seedlings were rarely significant and were not in agreement with ratings of disease severity on adult plants in the field. Based on measurements on older but still vegetative plants in the field, the gene or genes on the short arm of chromosome 4 and the long arm of chromosome 6 appeared to be related to a reduction in lesion numbers and an increase in incubation period, both of which were correlated with disease severity. Lesion length, lesion expansion rate, and sporulation intensity were not associated with partial resistance in Mo17. The lack of consistency in expression of components of partial resistance in seedling plants may be due to environmental and developmental regulation of genes for resistance.