GABAA receptor-associated protein (GABARAP) induces apoptosis by interacting with DEAD (Asp-Glu-Ala-Asp/His) box polypeptide 47 (DDX 47)
GABAA receptor-associated protein (GABARAP) induces apoptosis by interacting with DEAD (Asp-Glu-Ala-Asp/His) box polypeptide 47 (DDX 47)
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DOI：
10.1007/s10529-005-3628-2
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发表时间：
2005-05-01
影响因子：
2.7
通讯作者：
Chun, T
中科院分区：
文献类型：
--
作者：
Lee, JH;Rho, SB;Chun, T
GABA(A) 受体相关蛋白 (GABARAP) 是一种 14 kDa 的细胞质蛋白，最初被鉴定为皮质神经元中 GABA(A) 受体的分子伴侣。然而，有证据表明 GABARAP 的功能比神经元细胞中的特定作用要广泛得多。作为定义 GABARAP 生物学作用的第一步，我们使用免疫共沉淀结合 LC-MS/MS 分析来寻找 GABARAP 的结合伴侣。因此，最近被鉴定为 RNA 解旋酶的 DEAD (Asp-Glu-Ala-Asp/His) 盒多肽 47 (DDX 47) 被鉴定为 GABARAP 的结合配偶体。酵母双杂交系统进一步证实了 GABARAP 和 DDX 47 之间的相互作用。此外，将 GABARAP 和 DDX47 cDNA 共转染至肿瘤细胞系中可诱导细胞凋亡。这一结果可能是表明GABARAP和DDX 47参与细胞凋亡过程的第一个证据。
GABA(A) receptor-associated protein (GABARAP) is a 14-kDa cytoplasmic protein initially identified as a molecular chaperone for GABA(A) receptor in cortical neurons. However, evidence indicates that the function of GABARAP is much broader than a specific role in neuronal cells. As an initial step to define the biological role of GABARAP, we searched for binding partners of GABARAP using co-immunoprecipitation coupled with LC-MS/MS analysis. As a result, DEAD (Asp-Glu-Ala-Asp/His) box polypeptide 47 (DDX 47), recently identified as RNA helicase, is identified as a binding partner of GABARAP. An interaction between GABARAP and DDX 47 was further confirmed by yeast two-hybrid systems. Further, co-transfection of GABARAP and DDX47 cDNA into a tumor cell line induces apoptosis. This result may be the first evidence showing that GABARAP and DDX 47 are involved in the apoptotic process.