Cell behaviors and genetic lineages of the mesencephalon and rhombomere 1
Cell behaviors and genetic lineages of the mesencephalon and rhombomere 1
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DOI：
10.1016/j.neuron.2004.07.010
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发表时间：
2004-08-05
期刊：
影响因子：
16.2
通讯作者：
Joyner, AL
中科院分区：
文献类型：
--
作者：
Zervas, M;Millet, S;Joyner, AL
源自中脑 (mes) 和菱形核 1 (0) 的大脑结构调节不同的运动和感觉模式。然而，支撑 mes 和 r1 细胞结构组织的确切起源和细胞行为尚不清楚。在小鼠中使用一种新型诱导遗传命运图谱方法，我们以精细的时间和空间分辨率确定了 mes/r1 细胞的命运和谱系关系。我们证明 mes 和 r1 是神经节，它们与峡部组织者一起通过在 E8.5 和 E9.5 之间顺序建立的谱系限制边界沿前后轴划分。此外，源自最后部中枢的一小群细胞表现出前部室内扩张，并在整个下丘中发挥作用。最后，我们还发现了腹侧中脑多巴胺能和腹侧后脑血清素能神经元前体在 Wnt1 和 GO 表达方面的瞬时和差异遗传谱系。
Brain structures derived from the mesencephalon (mes) and rhombomere 1 (0) modulate distinct motor and sensory modalities. The precise origin and cellular behaviors underpinning the cytoarchitectural organization of the mes and r1, however, are unknown. Using a novel inducible genetic fate mapping approach in mouse, we determined the fate and lineage relationships of mes/r1 cells with fine temporal and spatial resolution. We demonstrate that the mes and r1 are neuromeres that along with the isthmic organizer are partitioned along the anterior-posterior axis by lineage restriction boundaries established sequentially between E8.5 and E9.5. Furthermore, a small group of cells originating from the most posterior mes exhibit anterior intracompartmental expansion and contribute throughout the inferior colliculus. Finally, we also uncovered transient and differential genetic lineages of ventral midbrain dopaminergic and ventral hindbrain serotonergic neuronal precursors with respect to Wnt1 and GO expression.