Estimation of the age of human bloodstains by electron paramagnetic resonance spectroscopy: Long-term controlled experiment on the effects of environmental factors
Estimation of the age of human bloodstains by electron paramagnetic resonance spectroscopy: Long-term controlled experiment on the effects of environmental factors
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DOI：
10.1016/j.forsciint.2005.02.029
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发表时间：
2005-08-11
影响因子：
2.2
通讯作者：
Sakurai, H
中科院分区：
文献类型：
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作者：
Fujita, Y;Tsuchiya, K;Sakurai, H
在这项研究中，我们研究了电子顺磁共振（EPR）的有效性和局限性，估计人类血痕的年龄。在77 K时，人血痕在g = 6.2（g6）、4.3（g4）、2.27（H）和2.005（R）区域分别产生了四个明显的EPR信号，分别是由于铁的高自旋、铁的非血红素、铁的低自旋和自由基物种。我们发现，绘制双曲线的EPR强度比H/G4与出血后的天数给出了一个线性相关性高达432天的误差范围内的25%的实际天数在受控条件下。然而，环境因素如吸收剂的差异，光照和储存温度的波动影响这些EPR活性化合物的变化，这导致错误估计的时间，因为出血发生。因此，在用这种方法估计自出血以来的时间时，应考虑这些因素。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
In this study, we examined the efficacy and limitations of electron paramagnetic resonance (EPR) for estimating the age of human bloodstains. At 77 K, human bloodstains give four striking EPR signals in the g = 6.2 (g6), 4.3 (g4), 2.27 (H) and 2.005 (R) regions due to ferric high-spin, ferric non-heme, ferric low-spin and free radical species, respectively. We found that plotting double logarithms of the EPR intensity ratio of H/g4 versus days past bleeding gave a linear correlation up to 432 days with an error range within 25% of the actual number of days under controlled conditions. However, environmental factors such as differences of absorbent, light exposure and fluctuations of storage temperature affected the changes of these EPR-active compounds, which result in misestimation of the time since bleeding occurred. Therefore, one should take such factors into account in estimating the period since bleeding by this method. (c) 2005 Elsevier Ireland Ltd. All rights reserved.