ENAC1, a NAC Transcription Factor, is an Early and Transient Response Regulator Induced by Abiotic Stress in Rice (Oryza sativa L.)
ENAC1, a NAC Transcription Factor, is an Early and Transient Response Regulator Induced by Abiotic Stress in Rice (Oryza sativa L.)
复制标题
ENAC1 是一种 NAC 转录因子，是水稻 (Oryza sativa L.) 非生物胁迫诱导的早期和瞬时响应调节因子
DOI：
10.1007/s12033-011-9477-4
复制
发表时间：
2012-10-01
影响因子：
2.6
通讯作者：
Zhang, Hong-Sheng
中科院分区：
文献类型：
--
作者：
Sun, Hui;Huang, Xi;Zhang, Hong-Sheng
植物特有的NAC（NAM、ATAF和CUC）结构域蛋白在植物发育和胁迫响应中起着重要作用。本研究从水稻中克隆了一个全长cDNA，命名为ENAC 1（early NAC-domain protein induced by abiotic stress 1）。ENAC 1在N端具有一个NAC结构域。比较时程表达分析表明，ENAC 1的表达，类似于OsDREB 1A，诱导非常迅速的各种非生物胁迫，包括盐，干旱，寒冷，外源脱落酸。然而，非生物胁迫对ENAC 1的诱导是短暂的，可持续3 h，而OsDREB 1A的诱导持续时间较长。ENAC 1的启动子序列含有几个顺式元件，包括阿坝响应元件，但不存在众所周知的脱水响应元件/C-重复元件。在水稻原生质体细胞中，ENAC 1-GFP（绿色荧光蛋白）融合蛋白定位于细胞核内。酵母杂交实验表明，ENAC 1是一个转录激活因子，与NAC识别序列（NACRS）结合。共表达分析表明，ENAC 1与一些胁迫相关基因共表达。总而言之，ENAC 1可能是应激反应的早期转录激活因子，并在非生物应激下调节NACRS介导的基因表达中发挥作用。
The plant-specific NAC (NAM, ATAF, and CUC)-domain proteins play important roles in plant development and stress responses. In this research, a full-length cDNA named ENAC1 (early NAC-domain protein induced by abiotic stress 1) was isolated from rice. ENAC1 possess one NAC domain in the N-terminus. Comparative time-course expression analysis indicated that ENAC1 expression, similar with OsDREB1A, was induced very quickly by various abiotic stresses including salt, drought, cold, and exogenous abscisic acid. However, the induction of ENAC1 by abiotic stress was transient and lasted up to 3 h, whereas that of OsDREB1A maintained longer. The promoter sequence of ENAC1 harbors several cis-elements including ABA response elements, but the well-known dehydration responsive element/C-repeat element is absent. The ENAC1-GFP (green fluorescent protein) fusion protein was localized in the nucleus of rice protoplast cell. Yeast hybrid assays revealed that ENAC1 was a transcription activator and bound to NAC recognition sequence (NACRS). Co-expression analysis suggested that ENAC1 co-expressed with a number of stress-related genes. Taken together, ENAC1 may be an early transcription activator of stress responses and function in the regulation of NACRS-mediated gene expression under abiotic stress.