Precise comparison of protoporphyrin IX fluorescence spectra with pathological results for brain tumor tissue identification
Precise comparison of protoporphyrin IX fluorescence spectra with pathological results for brain tumor tissue identification
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DOI：
10.1007/s10014-010-0002-4
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发表时间：
2011-02-01
影响因子：
3.3
通讯作者：
Sakuma, Ichiro
中科院分区：
文献类型：
--
作者：
Ando, Takehiro;Kobayashi, Etsuko;Sakuma, Ichiro
光动力诊断用于神经胶质瘤手术期间。尽管一些研究表明荧光光谱对于精确的肿瘤诊断是有效的，但以前表征光谱的方法一直存在问题，这可能导致误诊。在本文中，我们介绍了一种比较技术来表征病理学光谱和使用人脑组织进行初步测量的结果。我们开发了一种光谱扫描系统，可以对原始组织进行光谱测量。由于组织制剂保留了装置支架的形状，因此可以将光谱与病理检查进行精确比较。作为初步分析，我们测量了 5 名脑肿瘤患者的 13 个组织样本。该技术使我们能够测量光谱并将其与病理结果进行比较。一些组织在光谱和病理结果之间表现出良好的关系。虽然存在一些假阳性和假阴性的情况，但假阳性组织具有不同的光谱，其中短波长侧的强度也较高。所提出的技术提供了定量荧光光谱与病理结果的准确比较。我们发现频谱分析可以减少误报错误。这些结果将提高肿瘤组织鉴定的准确性。
Photodynamic diagnosis is used during glioma surgery. Although some studies have shown that the spectrum of fluorescence was efficient for precise tumor diagnosis, previous methods to characterize the spectrum have been problematic, which can lead to misdiagnosis. In this paper, we introduce a comparison technique to characterize spectrum from pathology and results of preliminary measurement using human brain tissues. We developed a spectrum scanning system that enables spectra measurement of raw tissues. Because tissue preparations retain the shape of the device holder, spectra can be compared precisely with pathological examination. As a preliminary analysis, we measured 13 sample tissues from five patients with brain tumors. The technique enabled us to measure spectra and compare them with pathological results. Some tissues exhibited a good relationship between spectra and pathological results. Although there were some false positive and false negative cases, false positive tissue had different spectra in which intensity of short-wavelength side was also high. The proposed technique provides an accurate comparison of quantitative fluorescence spectra with pathological results. We found that spectrum analysis may reduce false positive errors. These results will increase the accuracy of tumor tissue identification.