Studies on the Non-starchy Polysaccharides of the Endosperm of Naked Barley:Part II. The Periodate Oxidative Degradation of F-1 β-Glucan Prepared from the Endosperm of Naked Barley
Studies on the Non-starchy Polysaccharides of the Endosperm of Naked Barley:Part II. The Periodate Oxidative Degradation of F-1 β-Glucan Prepared from the Endosperm of Naked Barley
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裸大麦胚乳非淀粉多糖的研究：下部分：裸大麦胚乳中F-1β-葡聚糖的高碘酸盐氧化降解
DOI：
10.1080/00021369.1966.10858665
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发表时间：
1966
期刊：
影响因子：
--
通讯作者：
Y. Sakurai
中科院分区：
文献类型：
--
作者：
O. Igarashi;Y. Sakurai
大麦胚乳中的F-1β-葡聚糖是水溶性β-葡聚糖的主要成分，用环状芽孢杆菌的昆布多糖酶降解。该酶将F-1β-葡聚糖转化为三糖3-O-β-纤维二糖基-d-葡萄糖 * 和四糖3-O-β-纤维三糖基-d-葡萄糖 * 作为主要产物。这些产物占聚合物的74%，已通过化学方法鉴定。作为次要或痕量组分，检测到昆布二糖、纤维二糖、纤维三糖 ** 和两种未鉴定的四糖。结果表明，F-1β-葡聚糖主要由两种结构序列组成，一种是三聚体结构，其中一个β-（1→3）键与两个连续的β-（1→4）键交替排列;另一种是四聚体结构，其中一个β-（1→3）键与三个连续的β-（1→4）键交替排列。环水解1，3-连接的β-d-吡喃葡萄糖残基还原侧的葡萄糖键。
The F-1β-glucan from naked barley endosperm, a main component of water solubleβ-glucan, has been subjected to degradation with the laminarinase fromBacillus circulans. This enzyme converts the F-1β-glucan to a trisaccharide, 3-O-β-cellobiosyl-d-glucose*, and a tetrasaccharide, 3-O-β-cellotriosyl-d-glucose*, as the main products. These products, which constitute 74% of the polymer, have been identified by chemical methods. As the minor or trace components, laminaribiose, cellobiose, cellotriose** and two unidentified tetrasaccharides are detected. Overall data show that F-1β-glucan mainly consists of two types of structural sequences; one is trimeric unit in which a singleβ-(1→3) linkage alternates with two consecutiveβ-(1→4) linkage, and the other, a tetrameric unit in which a singleβ-(1→3) linkage alternates with three consecutiveβ-(1→4) linkages.It is shown that this laminarinase fromB. circulanshydrolyses the glucoside bond of the reducing side of 1,3-linkedβ-d-glucopyranose residues.